Tocotrienols as Anti-Chlamydials

Ann N Y Acad Sci

Tocotrienols

A while back, Dr. Stratton had sent me a few articles about tocotreinols. Periodically I get a slew of articles from him frequently without explanation in the email. I kind of take it as a test; it brings me back to my grad school habits, that my job is to figure out the significance of them to this whole Cpni project. I don’t think that he is trying to test me, but rather the Cpn links are obvious to him as he is so expert in the biochemistry and microbiological details. It doesn’t always occur to him that others may lack the advanced degree to discern his train of thought! Red (Dan), who has had conversations with Dr. Stratton regarding Cpn and rosaceai, has similarly reported having conversations with the good doctor where he has missed half the information just scrambling to take notes and keep up.

Usually I follow up sometime later in a phone call, confess my ignorance, and get explanation for the connections of the articles to Cpn if it’s not obvious. Well this time I clearly “failed the test” as I never really understood the importance of tocotrienols to our Cpn project, nor posted the references. In my defense, the particular articles were more general in nature and didn’t specifically refer to Cpn or infectionsi at all, so I saw it more as an FYI (For Your Information). Worse though, in retrospect, I actually confused tocotrienols with tocopherols, the more common Vit E supplement. Tocotreinols are not; repeat not the same as tocopherols. In fact, for our particular anti-chlamydial purposes, the tocopherols (regular Vitamin E) can interfere with the positive actions of the tocotreinols and should be taken hours apart.

Recently, while researching another topic entirely, I happened on a patent[i]<, and study,[ii]< which described the anti-chlamydial nature of tocotreinols, especially of delta and gamma tocotrienol (tocotrienols like Vit E come in alpha, delta and gamma forms). Subsequent review showed references on tocotrienols as anti-inflammatory, neuroprotective, lowering cholesteroli, countering lipid oxidation, anti-tumor, as they are apoptotic for a number of bacteria and for cancer cells, and other useful processes.

I decided to post this first in the forums, as it’s not at this point an “official” part of the CAPi protocolsi at this point. If I get a recommendation from one of the Cpn doc’s I’ll move it to the Handbook, but it seems useful to open it to community scrutiny and comment at this point. Cpnhelp readers can decide for themselves as to the potential usefulness of adding this supplement to their regime. PLEASE DON’T READ THIS AS A RECOMMENDED CURE ALL AND GET ALL ENTHUSIASTIC! Just check it out, and we’ll accumulate some forum responses to see how it affects the CAP as folks experiment.

Tocotrienol As Anti-Chlamydial Agent

The one that really got my attention is a patent, US 20060241174 titled “Vitamin E tocotrienols inhibition of intracellularly obligate pathogen Chlamydia and methods of use” by Mueller; Anne; (Sunderland, MA); Stuart; Elizabeth; (Amherst, MA); Tan; Barrie; (Amherst, MA).

Dr. Stuart is a leading Chlamydiologist doing stellar work in this area along with her grad students. She is the author of the study, which found a significant percentage of viable Cpn in healthy blood donors referred to elsewhere on this site.[iii]< The research in the patent is impeccable science to my reading, and worth a read to the technically minded. It is much like the Vanderbilt patents by Mitchell and Stratton in the clarity of explanation and thoroughness of the research. I will try to give a brief summary and let readers look it up themselves for the full read.

Using (delta & gamma) tocotrienol:

· To inhibit, prevent, and disrupt the developmental cell cycle and infection of Chlamydia” [iv]<

· Reduce the number of chlamydial inclusions into host cells (the means by which Cpn enters the host cell) by tocotrienol’s interference with the use of cell membrane cholesterol by Cpn.

· Impede and suppress the initial infection by EB’s (the infectious Chlamydial Elementary Bodies).

· To inhibit development and replication of Chlamydia within the host cell.

Their work is specifically directed:

· At Chlamydia in circulating WBCs, including neutrophils, monocytes, lymphocytes, eosinophils, and basophils.

· To reduce the cholesterol level and chlamydial infection in hyperlipidemic patients

· To restrict cholesterol/lipids to restrict Chlamydia infection and growth.

· Reduce Chlamydia infections in cancer patients.

· To ward off pathogenic infections including Chlamydia via activation of antigen-presenting dendritic cellsi and T-lymphocytes.

· To resist opportunistic chlamydial infection of hypertensive patients, and thereby reduce the patient's blood pressure.

· To reduce cardiovascular risk factors associated with metabolic syndrome, and thereby reduce the risk of diabetes and concomitant or subsequent infection by Chlamydia.

Basically their research suggests that the tocotrienols interference in both the process by which Chlamydia enters the cell via hypothesized “lipid rafts,” and in the process by which Chlamydia hijacks cholesterol from the cells lipid transport mechanisms. Like ATP, Chlamydia cannot manufacture cholesterol itself, and but requires it for it’s own survival and replication. Tocotrienols appear to inhibit the entry of EB’s into the host cell, inhibit the conversion of EB’s to RB’s as well as the replication process of RB’s to EB’s. It does not, to my understanding of their explanation, does not prevent or limit the formation of the aberrant cryptic forms, although it appears to induce apoptosis of infected host cells as well as of tumor cells—both types of cells are highly cholesterol dependant.

This appears to be a pretty multifaceted supplement in regards to Chlamydia. And that doesn’t even cover the neuroprotective and other useful effects.

One of things I found in researching further is that it is critical to have a high delta, high gamma form of tocotrienol, and also that it not have any tocopherols which would interfere with it’s effects. Annatto is one of the few origins of tocotreinols that fit this profile. Note that the authors also have patented a proprietary method of annatto extraction to produce this kind of tocotrienol.

An overview article[v]< notes that tocotrienol overall:

· Most powerful antioxidant">i of the vitamin E family.

· The most potent tocotrienol to activate anticancer effects.

· More effective at accumulating in cells compared to other tocotrienols.

· Most effective member of the vitamin E for reducing endothelial expression of adhesion molecules, thereby preventing the accumulation of inflammatory cells within the arterial wall.

· Most potent tocotrienol in inducing apoptosis (cell deaths) of human breast cancer cells. Delta-tocotrienol was twice as potent as of gamma-tocotrienol.

· Inhibits the excessive aggregation of blood platelets much more effectively than vitamin E or other tocotrienols.

Neuroprotective Effects-

Tocotrienols appear to exert significant neuroprotective affects, by both anti-oxidant and non-anti-oxidant pathways.[vi]< Dietary tocotrienols do reach the brain, and so would be expected to exert this effect in brain tissue.[vii]< Tocotrienols protected the brain against stroke- and glutamate-induced degeneration in rats.

Immunei Enhancing, Anti-viral and Anti-Cancer Effects-

Tocotrienols and alpha tocopherol have been shown to increase Ig production in the spleen and mesentery lymphatics, and to regulate immune function as well as to affect the proliferation and function of spleen and MLN lymphocyte. [viii]< Tumor inhibiting effects[ix]< [x]< as well as tumor inhibition specific to prostatei cancer cell lines (guys, pay attention!)[xi]< have also been found. This same study hints at antiviral effects of tocotrienols as they inhibited by inhibiting EBVi EA expression

Cardiac Effects-

It is in the area of cardiac disease that the interest in tocotreinols has perhaps been highest. This appears to be the result of tocotrienols effect on lipid profiles and on oxidation of lipids as well as on the association of Chlamydia pneumonia with artherosclerotic plaques[xii]< and the tocotrienol’s effect on Cpn as demonstrated by Muller, et al. There are a number of useful reviews that detail this highly researched area.[xiii]< [xiv]< Additionally, they have impact on blood sugar and diabetes.[xv]<

Safety & Interactions

Dosage and safety cautions are sparser to come by. Delta-tocotrienol - The 21st Century Vitamin E? (ibid) notes:

What are the dosage, drug interactions, and safety information for tocotrienols?
 The effectiveness of tocotrienols for some specific indications requires using tocopherol-free, high delta-tocotrienol products at a proper dosage. For example, for lowering cholesterol, utilize the 90% delta-tocotrienol preparation and begin with a dosage of 50 mg tocotrienols. Recheck cholesterol levels in four to six weeks and alter dosage accordingly. If cholesterol levels drop into the normal range, the dosage can be reduced. If cholesterol levels do not change enough, the dosage can be increased to 100 mg per day. For best results take the tocotrienols with food and at least one hour away from any vitamin E.

Tocotrienols are extremely safe and no side effects have been reported.13 Given the effects on platelet aggregation, you will need to inform your physician of their use if you are going in for surgery, or taking the blood thinning drug Coumadin® (warfarin) or anti-platelet drugs like Ticlid® (ticlopidine).

However, doses of the form mentioned in the anti-Chlamydial patent range from 10 mg and 1000 mg per day. They also note:

It is likely that control and eradication of Chlamydia in humans will require tocotrienol supplementation for an extended period. In light of this, tocotrienols and other agents (such as tangeretin, nobiletin, EGCG, and resveratol; more in Example 7) in monotherapies or in combination are superior because of their lack of toxicity. In contrast, other drugs (such as statins) have sustained toxicities with chronic usage. (ibid)

We also don’t know to what degree use of tocotrienols produces die-off reactions or secondary porphyriai increases. Certainly, the inhibition of EB inclusions should not produce any die-off reactions unless it renders EB’s trying to enter the cell more vulnerable to the immune system. You’d expect not much more than the “NAC flu” type of reaction, if that. Inhibition of conversion of EB’s to RB’s as well as of RB replication processes should also not create much reaction, depending on how much LPSi or HSP60 endotoxini is being generated by Cpn during these processes. However, the apoptotic inducing features of tocotrienol could potentially generate secondary porphyria reactions if the bursting of host cells releases intracellular stores of accumulated porphyrins. My suspicion is that the latter occurs at higher dosing, but of course we don’t really know what higher dosing means.

So, dear readers, if any of you experiment with this supplement we expect a detailed report of your findings. Remember, it is the high delta-tocotrienol form that you will need to look for, and make sure it is not mixed with tocopherols, which you should probably be taking too but at a widely separated time. And you thought your med schedule was complicated already!


[i]< By the way, if you are a Macintosh user there is a wonderful little free program called PatentDownloader which not only can searh both US and World patents, but downloads them, along with associated pictures and charts! Plus it has the cutest kitty icon. Whimsy is very important when doing research! http://www.oneriver.jp/PD/<

[ii]< D-199. Impact of Hypocholesterolemic Delta-Tocotrienol on Chlamydial Development In Vitro

A. M. Mueller1, B. Tan2, E. S. Stuart1;

http://ieg.ou.edu/ASM2006/data/papers/D_199.htm<

University of Massachusetts, Amherst, MA, 2American River Nutrition, Inc., Hadley, MA.

Chlamydiae, inclusion-forming obligate intracellular bacteria, are associated with common pathologies including Alzheimer’s disease, atherosclerosis, coronary heart diseasei, asthmai, and respiratory tract infections. Entry by Chlamydiae, including C. pneumoniae and most C. trachomatis genital tract serovars, involves cholesterol-rich lipid raft domains. These can contain caveolae, a key element in cholesterol homeostasis with which Chlamydia may associate at cholesterol-enriched plaque regions. We hypothesized that the hypocholesterolemic activity of delta-tocotrienol, a vitamin E compound, might impact infection by Chlamydia. Human mammary tumor (MCF-7, TMX2-28), epithelial (HEp-2), B-lymphocyte (JY), and murine macrophage (J774A.1) cell lines were incubated with delta-tocotrienol concentrations of 10-30 μmol/L for 6 h prior to infection by serovar K, a C. trachomatis strain of the sexually transmitted disease biovar. At intervals between 24-72 h, cells were fixed, then samples were assessed, and infected cells identified by immunofluorescent staining followed by quantitative flow cytometry and confocal microscopy. Detected infection levels for cells pretreated with delta-tocotrienol were decreased by >50%. In addition, confocal immunofluorescent microscopy demonstrated the concomitant occurrence of aberrant pathogen inclusion development, including an apparent failure of fusion among the individually internalized elementary body clusters, and the formation of notably smaller inclusions. Microscopic counts of large and small inclusions in the delta-tocotrienol vs. control cells showed decreases of 3- and 2-fold, respectively. For JY cells, flow cytometry showed that a decrease of Chlamydia-infected cells was at least 2-fold during an infection period of 72 h, with a 2.6-fold maximum at 36 h. Since lipids and lipid levels may be critical to Chlamydia infections in vivo, hyperlipidemic patients were assessed for blood cell borne Chlamydiae. The in vivo impact of dietary delta-tocotrienol on Chlamydia carriage is under examination in a clinical study with cholesterol-suppressive delta-tocotrienol.

[iii]< http://www.bio.umass.edu/micro/faculty/stuart.html<

The pictures on her lab webpage of chlaydia inclusions stained with florescent dye are worth a look, as are those on http://www.bio.umass.edu/micro/faculty/webley.html< the page of Dr. Webley.

[iv]< From the patent abstract: “Chlamydial infection levels in mouse macrophages treated with tocotrienol were decreased >50%, with concomitant aberrant pathogen development. The number of large and small inclusions in tocotrienol-versus-control cells was decreased 3-fold and 2-fold, respectively. When treated with delta tocotrienol, Chlamydia in human lymphocytes was inhibited by at least 2.6-fold in 1.5 days. Dietary delta tocotrienol inhibited Chlamydia infection and persistencei in hypercholesterolemic patients with a corresponding drop in LDL. These studies demonstrate that tocotrienol lowers cholesterol, thus preventing or diminishing the cholesterol hijacking by Chlamydia obligatory for its infectivity and replication. Therefore, hypolipidemic agents used to treat cardiovascular diseasesi, metabolic syndrome, and diabetes are used as monotherapies, or in combination with tocotrienol to treat Chlamydia.”

[v]< Delta-tocotrienol - The 21st Century Vitamin E? http://www.doctormurray.com/newsletter/1-06-2003.htm<

Tocotrienols Molecular Siblings of Tocopherols with Unique Vitamin E Properties

By Geoffrey C. Kwiat, Ph.D. http://www.vrp.com/articles.aspx?ProdID=art340&zTYPE=2<

[vi]< J Neurochem. 2006 September; 98(5): 1474–1486.

doi: 10.1111/j.1471-4159.2006.04000.x.

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1847628<

Characterization of the potent neuroprotective properties of the natural vitamin E α-tocotrienol

Savita Khanna, Sashwati Roy, Narasimham L. Parinandi, Mariah Maurer, and Chandan K. Sen

Savita Khanna, Laboratory of Molecular Medicine, Davis Heart & Lung Research Institute, The Ohio State University Medical Center, Columbus, Ohio 43210.

All author affiliations.

Corresponding Author: Prof. Chandan K. Sen 512 Davis Heart & Lung Research Institute 473 West 12™ Avenue The Ohio State University Medical Center Columbus, Ohio 43210 Tel. 614 247 7658 Fax 614 247 7818 Email: chandan.sen@osumc.edu

The natural vitamin E tocotrienols possess properties not shared by tocopherols. Nanomolar alpha-tocotrienol, not alpha-tocopherol, is potently neuroprotective (JBC 275:13049; 278:43508; Stroke 36:2258). On a concentration basis, this finding represents the most potent of all biological functions exhibited by any natural vitamin E molecule. We sought to dissect the antioxidant-independent and –dependent neuroprotective properties of alphatocotrienol by using two different triggers of neurotoxicity, homocysteic acid (HCA) and linoleic acid. Both HCA and linoleic acid caused neurotoxicity with comparable features such as increased GSSG/GSH, elevated [Ca2+]i and compromised mitochondrial [Delta]ψ . Mechanisms underlying HCA-induced neurodegeneration were comparable to the path implicated in glutamate-induced neurotoxicity. Inducible activation of c-Src and 12-lipoxygenase (12-Lox) represented early events in that pathway. Over-expression of active c-Src or 12-Lox sensitized cells to HCA-induced death. Nanomolar alpha-tocotrienol protected. Knock-down of c-Src or 12-Lox attenuated HCA-induced neurotoxicity. Oxidative stress represented a late event in HCA-induced death. The observation that micromolar, but not nanomolar, alpha-tocotrienol functions as an antioxidant was verified in the model involving linoleic acid induced oxidative stress and cell death. Oral supplementation of alpha-tocotrienol to humans results in a peak plasma concentration of 3 micromolar. Thus, oral alpha-tocotrienol may be neuroprotective by antioxidant-independent as well as antioxidant-dependent mechanisms.

[vii]< Tocotrienol: the natural vitamin E to defend the nervous system?

Sen CK<, Khanna S<, Roy S<.

http://www.annalsnyas.org/cgi/content/abstract/1031/1/127<

Davis Heart & Lung Research Institute, 473 West 12th Avenue, The Ohio State University Medical Center, Columbus, Ohio 43210, USA. sen-1@medctr.osu.edu

Vitamin E is essential for normal neurological function. It is the major lipid-soluble, chain-breaking antioxidant in the body, protecting the integrity of membranes by inhibiting lipid peroxidation. Mostly on the basis of symptoms of primary vitamin E deficiency, it has been demonstrated that vitamin E has a central role in maintaining neurological structure and function. Orally supplemented vitamin E reaches the cerebrospinal fluid and brain. Vitamin E is a generic term for all tocopherols and their derivatives having the biological activity of RRR-alpha-tocopherol, the naturally occurring stereoisomer compounds with vitamin E activity. In nature, eight substances have been found to have vitamin E activity: alpha-, beta-, gamma- and delta-tocopherol; and alpha-, beta-, gamma- and delta-tocotrienol. Often, the term vitamin E is synonymously used with alpha-tocopherol. Tocotrienols, formerly known as zeta, , or eta-tocopherols, are similar to tocopherols except that they have an isoprenoid tail with three unsaturation points instead of a saturated phytyl tail. Although tocopherols are predominantly found in corn, soybean, and olive oils, tocotrienols are particularly rich in palm, rice bran, and barley oils. Tocotrienols possess powerful antioxidant, anticancer, and cholesterol-lowering properties. Recently, we have observed that alpha-tocotrienol is multi-fold more potent than alpha-tocopherol in protecting HT4 and primary neuronal cells against toxicity induced by glutamate as well as by a number of other toxins. At nanomolar concentration, tocotrienol, but not tocopherol, completely protected neurons by an antioxidant-independent mechanism. Our current work identifies two major targets of tocotrienol in the neuron: c-Src kinase and 12-lipoxygenase. Dietary supplementation studies have established that tocotrienol, fed orally, does reach the brain. The current findings point towards tocotrienol as a potent neuroprotective form of natural vitamin E.

[viii]< Biosci Biotechnol Biochem (1999) 63: 1697-702.

Dietary effect of tocopherols and tocotrienols on the immune function of spleen and mesentery

http://grande.nal.usda.gov/ibids/index.php?mode2=detail&origin=ibids_references&therow=319704<

JY Gu, Y Wakizono, Y Sunada, P Hung, M Nonaka, M Sugano, K Yamada

Department of Bioscience and Biotechnology, Kyushu University, Fukuoka, Jap

The immunoregulatory effects of dietary alpha-tocopherol (Toc) and tocotrienols (T-3) on humoral and cell-mediated immunity and cytokinei productions were examined in Brown Norway rats. We found that the IgA and IgG productivity of spleen and mesenteric lymph node (MLN) lymphocytes was significantly enhanced in the rats fed on Toc or T-3, irrespective of concanavalin A (Con A) stimulation of the lymphocytes. On the contrary, the IgE productivity of lymphocytes from the rats fed on Toc or T-3 was less without Con A stimulation, but was greater in the presence of Con A, especially in the T-3 group. Toc or T-3 feeding significantly decreased the proportion of CD4+ T cells and the ratio of CD4+/CD8+ in both spleen and MLN lymphocytes of the rats fed on Toc or T-3. The interferon-gamma productivity of MLN lymphocytes was higher in the rats fed on Toc or T-3 than in those fed on a control diet in the presence of Con A, while that of spleen lymphocytes was lower in the rats fed on Toc or T-3. In addition, T-3 feeding decreased the productivity of tumor necrosisi factor-alpha of spleen lymphocytes, while it enhanced the productivity of MLN lymphocytes. These results suggest that oral administration of Toc and T-3 affects the proliferation and function of spleen and MLN lymphocytes.

[ix]< Ann N Y Acad Sci.< 2004 Dec;1031:127-42

URL: http://www.interscience.wiley.com/jpages/0020-7136/<

Title:Inhibition of tumour promotion by various palm-oil tocotrienols.

Author:Goh, S H : Hew, N F : Norhanom, A W : Yadav, M

Citation:Int-J-Cancer. 1994 May 15; 57(4): 529-31

Abstract:

Inhibition of tumour promotion by various vitamin E compounds (tocopherols and

tocotrienols) and some of their dimers was examined by an in vitro assay utilizing the

activation of Epstein-Barr virus (EBV) early antigen (EA) expression in

EBV-genome-carrying human lymphoblastoid cells. The results reveal that gamma- and

delta-tocotrienols derived from palm oil exhibit a strong activity against tumour

promotion by inhibiting EBV EA expression in Raji cells induced by

12-O-tetradecanoylphorbol-13-acetate (TPA). However, alpha- and gamma-tocopherols

and dimers of gamma-tocotrienol or gamma-tocopherol lack this activity.

[x]< Cancer Lett. 2005 Nov 18;229(2):181-91. Epub 2005 Aug 10.Click here to read Links

Tumor suppressive effects of tocotrienol in vivo and in vitro.<

Wada S, Satomi Y, Murakoshi M, Noguchi N, Yoshikawa T, Nishino H.

Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-0841, Japan. poisson@koto.kpu-m.ac.jp

Tocotrienols have been reported to have higher biological activities than tocopherols. We investigated the antitumor effect of tocotrienols both in vivo and in vitro. Oral administration of tocotrienols resulted in significant suppression of liver and lung carcinogenesis in mice. In human hepatocellular carcinoma HepG2 cells, delta-tocotrienol exerted more significant antiproliferative effect than alpha-, beta-, and gamma-tocotrienols. delta-Tocotrienol induced apoptosis, and also tended to induce S phase arrest. On the other hand, gene expression analysis showed that delta-tocotrienol increased CYP1A1 gene, a phase I enzyme. Although further study will be necessary to investigate possible adverse effect, the data obtained in present study suggest that tocotrienols could be promising agents for cancer prevention.

[xi]< Tocotrienols and Prostate Cancer<

Authors: William L. Stone; K. Krishnan; Sharon Campbell; EAST TENNESSEE STATE UNIV JOHNSON CITY

Abstract: In this study we demonstrated that vitamin E isoforms, tocopherols and tocotrienols, have variable growth inhibitory effects on both types of prostate cancer cell line models. The gamma isoforms are more effective than the alpha isoforms and the tocotrienols are more effective than the tocopherols. This study further showed that the vitamin E-mediated inhibition of cell proliferation is preferential for cancer cells at concentrations of about 40 M or lower. Delta-tocotrienol (DT3), in particular, is infective against normal prostate epithelial cells but highly effective against LNCaP cancer cells. Collectively, our data supports the view that tocotrienols, particularly DT3 may prove very useful as chemotherapeutic or chemopreventive agents for treating prostate cancer. Our next will be to initiate experiments in animal models and then to initiate clinical studies.

Biochem Biophys Res Commun. 2006 Jul 28;346(2):447-53. Epub 2006 Jun 2.< Tocotrienol-rich fraction of palm oil induces cell cycle arrest and apoptosis selectively in human prostate cancer cells.

Srivastava JK, Gupta S.

Department of Urology, Case Western Reserve University, Cleveland, OH 44106, USA.

One of the requisite of cancer chemopreventive agent is elimination of damaged or malignant cells through cell cycle inhibition or induction of apoptosis without affecting normal cells. In this study, employing normal human prostate epithelial cells (PrEC), virally transformed normal human prostate epithelial cells (PZ-HPV-7), and human prostate cancer cells (LNCaP, DU145, and PC-3), we evaluated the growth-inhibitory and apoptotic effects of tocotrienol-rich fraction (TRF) extracted from palm oil. TRF treatment to PrEC and PZ-HPV-7 resulted in almost identical growth-inhibitory responses of low magnitude. In sharp contrast, TRF treatment resulted in significant decreases in cell viability and colony formation in all three prostate cancer cell lines. The IC(50) values after 24h TRF treatment in LNCaP, PC-3, and DU145 cells were in the order 16.5, 17.5, and 22.0 microg/ml. TRF treatment resulted in significant apoptosis in all the cell lines as evident from (i) DNA fragmentation, (ii) fluorescence microscopy, and (iii) cell death detection ELISA, whereas the PrEC and PZ-HPV-7 cells did not undergo apoptosis, but showed modestly decreased cell viability only at a high dose of 80 microg/ml. In cell cycle analysis, TRF (10-40 microg/ml) resulted in a dose-dependent G0/G1 phase arrest and sub G1 accumulation in all three cancer cell lines but not in PZ-HPV-7 cells. These results suggest that the palm oil derivative TRF is capable of selectively inhibiting cellular proliferation and accelerating apoptotic events in prostate cancer cells. TRF offers significant promise as a chemopreventive and/or therapeutic agent against prostate cancer.

: Bioorg Med Chem. 2006 Apr 15;14(8):2684-96. Epub 2005 Dec 27 <

Synthesis and study of the cancer cell growth inhibitory properties of alpha-, gamma-tocopheryl and gamma-tocotrienyl 2-phenylselenyl succinates.

Vraka PS, Drouza C, Rikkou MP, Odysseos AD, Keramidas AD.

University of Cyprus, Department of Chemistry, 1678 Nicosia, Cyprus.

Vitamin E succinate selenium-conjugated molecules were synthesized and their apoptogenic properties were evaluated. 4-Methyl-2-phenylselenyl succinate (4) was prepared by the reaction of sodium benzeneselenolate with 2-bromosuccinic anhydrite in methanol solution. The methyl ester was converted to the acid (5) by hydrolysis with aqueous hydrochloric acid. Reaction of the 2-phenylselenyl succinic anhydrite (6) with alpha-tocopherol (1a), gamma-tocopherol (1c), and gamma-tocotrienol (2c) in acidic conditions gave the respective esters. The free radical scavenging properties of alpha-tocopheryl-2-phenylselenyl succinate (7), gamma-tocopheryl-2-phenylselenyl succinate (8), and gamma-tocotrienyl-2-phenylselenyl succinate (9) were evaluated in comparison with those of alpha-tocopheryl succinate (10), gamma-tocopheryl succinate (11), and gamma-tocotrienyl succinate (12), respectively, and the free tocopherols and gamma-tocotrienol. Compounds 7-9 induced a statistically significant decrease in prostate cancer cell viability compared to 10-12, respectively, or 5, exhibiting features of apoptotic cell death and associated with caspase-3 activation. These data show that structural modifications of vitamin E components by 5 enhance their apoptogenic properties in cancer cells.

Ann N Y Acad Sci. 2004 Dec;1031:391-4.<

Gamma-tocotrienol metabolism and antiproliferative effect in prostate cancer cells.

Conte C, Floridi A, Aisa C, Piroddi M, Floridi A, Galli F.

University of Perugia, Department of Internal Medicine, Section of Applied Biochemistry and Nutritional Sciences, Via del Giochetto, 06126 Perugia, Italy. f.galli@unipg.it

In this study, we evaluated the antiproliferative effect of tocotrienols (T3) and the presence of a specific vitamin E metabolism in PC3 and LNCaP prostate cancer cells. These cell lines are able to transform tocopherols (T) and T3 in the corresponding carboxyethyl-hydroxychromans metabolites (CEHCs). The extent of this metabolism and the inhibitory effect on cell growth followed the order of magnitude alpha-T<alpha-T3<gamma-T<gamma-T3. The partial inhibition of gamma-T3 metabolism by ketoconazole did not influence cell proliferation. These early findings may suggest that the transformation of vitamin E to CEHC is mostly a detoxification mechanism useful to maintain the malignant properties of prostate cancer cells.

[xii]< J Nutr. 2001 Oct;131(10):2606-18.Click here to read Links

Novel tocotrienols of rice bran inhibit atherosclerotic lesions in C57BL/6 ApoE-deficient mice.

http://www.ncbi.nlm.nih.gov/pubmed/11584079<

Qureshi AA, Salser WA, Parmar R, Emeson EE.

Advanced Medical Research, Madison, WI 53719, USA. nqureshi@mhub.facstaff.wisc.edu

We are studying novel tocotrienols, which have a number of activities that might interfere with the formation of atherosclerotic plaques, including hypocholesterolemic, antioxidant, anti-inflammatory and antiproliferation effects. This study compared the effects of alpha-tocopherol, the tocotrienol-rich fraction (TRF(25)) and didesmethyl tocotrienol (d-P(25)-T3) of rice bran on the pathogenesis of atherosclerotic lesions in C57BL/6 apolipoprotein (apo)E-deficient (-/-) mice. These mice are an excellent model because they become hyperlipidemic even when they consume a low fat diet and they develop complex atherosclerotic lesions similar to those of humans. These compounds were also tested in wild-type C57BL/6 apoE (+/+) and (+/-) mice fed low or high fat diets. When a high fat diet was supplemented with alpha-tocopherol, TRF(25) or d-P(25)-T3 and fed to mice (+/+) for 24 wk, atherosclerotic lesion size was reduced 23% (P = 0.33), 36% (P = 0.14) and 57% (P < 0.02), respectively, and in mice (+/-) fed for 18 wk, lesions were reduced by 19% (P = 0.15), 28% (P < 0.01) and 33% (P < 0.005), respectively, compared with mice fed a control diet. A low fat diet did not cause atherosclerotic lesions in these mice. The low fat diet supplemented with TRF(25) or d-P(25)-T3 fed to apoE-deficient (-/-) mice for 14 wk decreased atherosclerotic lesion size by 42% (P < 0.04) and 47% (P < 0.01), respectively, whereas alpha-tocopherol supplementation resulted in only an 11% (P = 0.62) reduction. These results demonstrate the superior efficacy of tocotrienols compared with alpha-tocopherol. Although tocotrienols decreased serum triglycerides, total and LDL cholesterol levels, the decreases in atherosclerotic lesions seem to be due to the other activities. Serum tocol concentrations in various groups are also described. This is the first report of a significant reduction in the atherosclerotic lesion size in all three genotypes of apoE mice fed a novel tocotrienol (d-P(25)-T3) of rice bran. Dietary tocotrienol supplementsi may provide a unique approach to promoting cardiovascular health.

[xiii]< Altern Med Rev. 2001 Jun;6(3):248-71<.

Cardiovascular disease: C-reactive protein and the inflammatory disease paradigm: HMG-CoA reductase inhibitors, alpha-tocopherol, red yeast rice, and olive oil polyphenols. A review of the literature.

Patrick L, Uzick M.

The current understanding of the origin of atherosclerosis is that of an inflammatory process that involves the acute phase response -an innate biological response to a disturbance in homeostasis -infection, inflammationi, tissue injury, neoplasm, or immune disturbance. The activation of the acute phase response, signaled by interleukin-6, produces proteins (fibrinogen, C-reactive protein (CRP), serum amyloid A) that lead to inflammatory reactions. The tissues themselves contain elevated levels of acute phase proteins and cytokinesi resulting in a localized inflammatory effect. Localized inflammatory responses in the intimal layer of the arterial wall have been shown to be responsible for many of the aspects of intimal thickening and plaque disruption, leading to acute cardiovascular events. The predictive value of plasma C-reactive protein as a risk factor for cardiovascular events has led some researchers to support the use of CRP as a main cardiovascular risk assessment tool, along with total cholesterol:HDL ratios and homocysteinei levels. The ability of HMG-CoA reductase inhibitors to lower C-reactive protein levels has recently brought into question the mechanisms of action of the statin drugs. Because these medications lower incidences of acute cardiovascular events as well as decreasing morbidity and mortality well before the effects of lowered LDL cholesterol can be expected to occur, questions have been asked about whether they may work independently of LDL-lowering mechanisms. Red yeast rice contains a naturally-occurring statin (lovastatin) as well as other cholesterol-lowering compounds, some with antioxidant effects. Alpha-tocopherol also significantly lowers CRP levels in diabetics and nondiabetics, and minimizes other aspects of the acute phase response and inflammatory damage involved in atherosclerosis. This may account for alpha-tocopherol's positive effect on cardiovascular morbidity and mortality. Finally, polyphenolic compounds present in virgin olive oil also have anti-inflammatory and antioxidative effects in cardiovascular disease. The phenolic compounds in virgin olive oil may explain some of the protective effects found in epidemiological studies.

[xiv]<Annatto Tocotrienols:Vitamin E Component Dramatically More Effective at Supporting Heart Health

By Barrie Tan, PhD and Anne Mueller, MSc http://www.vrp.com/articles.aspx?ProdID=art2147&zTYPE=2<

[xv]< Annatto Tocotrienols: Their Significant Role in Blood Sugar Control

By John Raimo, MS, RD http://www.vrp.com/articles.aspx?ProdID=art2169&zTYPE=2<

Well, there you go another vitamin comes to the fore. One day our doctors will catch on. Thanks Jim I'll look ino it.

Michèle (UK) GFAi: Wheldon CAPi 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse. Zoo keeper for Ella, RRMSi, At worse EDSSi 9, 3 months later 7 now 5.5 Wheldon CAP 16th March 2006

Michèle (UK) GFAi: Wheldon CAPi 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse.

Okay Jim I will bite. I am confused on which one to buy at the store. Sooo since you have done the research I though I would take the easy way out and ask you. Which one to get and to take it with food or without. Funny that you say Dr.S gets to scientific my Dr. said the same thing when she spoke with him. Some of the conversation was way over her head and coming from a Dr. it kind of makes us feel not so bad. sjogren's diagnosed 2/03, 200mg minocin daily, mwf zithromax, flagyli every 3 weeks.

200mg doxyi daily, 500 zithromax mwf,flagyli 1000 m-fri.rifampin 2x daily,chloestryramine 2x daily

Hummm, ok, so who is going to be the guinea pig?? I cant right now. I'm slowly increasing my NACi to 5000mg. I'm congested for past 3 weeks but our weather here has been crazy, 30 to 40 degrees variance in a day or so. But once I'm on NAC 5000mg and no more congestion, either due to weather or NAC, I sure will.

I cant tolerate more than 1000mg of vit D a day without going into severe depression. It will be interesting to see what kind of die off this will produce.

Also, do you think that this might eventually replace one of the antibioticsi in our protocol (like NAC replaced Amoxicillini)? of course, opinions will be based upon speculation only.

Mphs, TN. CFSi, hypoT (Hashi), weak adrenals, 37 w/hormones of 80 yo. right arm neuropathy. + for cpni, myco, EBVi, CMV. on NAC 3600mg, doxyi 100-2xday, azith 250 m/w/f/sun, estriol, progesterone, synthroid, and pulsing w/flagyli.

Mphs, TN. CFSi, hypoT (Hashi), adrenal fatigue, hormonal inbalance. right arm neuropathy-getting better. cpni, myco, EBVi, CMV, HHV-6. Capi began in 6/07. NACi 2400mg, minoi 100mg bidi, biaxin 500mg bidi. cytomel, flagyli bid continuously.

A few minutes ago I would have to have admited defeat at finding tocotienols on their own, they came together with tocopherols in all the sites I visited, (about 6) but I found this< at iherb.com. The secret is to google for Annatto tocotrienols.

Michèle (UK) GFAi: Wheldon CAPi 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse. Zoo keeper for Ella, RRMSi, At worse EDSSi 9, 3 months later 7 now 5.5 Wheldon CAP 16th March 2006

Michèle (UK) GFAi: Wheldon CAPi 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse.

I started getting this version of CoQ10 from Vitacost: http://www.vitacost.com/products/ToCoQ10.cfm<

TocoQ10™ utilizes only the more effective 100% Japanese pharmaceutical grade natural trans-form CoQ10

NSI®'s ToCoQ10™ formula also contains a unique tocotrienol complex, Tocomin® SupraBio™, derived from the fruit oil of palm trees.

• Coenzyme Q10 (CoQ10), tocotrienols and tocopherols (potent forms of vitamin E) promote healthy heart function, provide powerful antioxidant">i protection and support the immunei system in its daily defense of the body.

• NSI® ToCoQ10™ contains supremely high-quality forms of both CoQ10 and tocotrienols. Our CoQ10 is 100% natural, produced using a patented method that results in a CoQ10 identical to the body's own.

• Contains rice bran oil, which provides additional cholesteroli health benefits and is rich in antioxidantsi (with one to two percent gamma-oryzanol and tocotrienols)

So perhaps if it wasn't for the fact that I had finished the protocol, I could be the guinea pig................Sarah

An Itinerary in Light and Shadow...........Completed Stratton/Wheldon regime for aggressive secondary progressive MS in June 2007, after four years, three of which intermittent.   Still slowly improving and no exacerbation since starting. EDSSi was 7, now 2, less on a good day.
Completed Stratton/Wheldon regime for aggressive secondary progressive MSi in June 2007, after four years, three of which intermittent.   Still improving bit by bit and no relapses since finishing treatment.

 I have some on order, so I'll be a guinea pig soon. It is critical that the form be high delta-tocotrienol, and that it not have any tocopherol (regular E). Supposedly the best form of this is derived from annatto as it has lot's of toco and what little E is in it can be distilled out. One brand: Delta Fraction Tocotrienols by Nutricology. I think Allergy Research also has a brand.

 

iHerb was out of stock so I ordered it elsewhere, but won't post the place until I find out how their service is, i.e. I had one order from a company and no response to my order, have tried another but haven't yet received the order. We'll see.

Careful not to get "tocotrienol complex" which often means it's a combo of tocotrienol and tocopherol. Sarah, you might want to find out if your supplement has the tocopherol mixed in, as the term "complex" is ambiguous-- sometimes means the combo of tocotrienols and sometimes the combo with tocopherols. 

 

CAPi for Cpni 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, Cytotec 100mg, Plaquenil 100mg, Magnascent Iodine 12 drps/day, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Great stuff, Jim. In doing some research for my brother a little while ago, I ran across this article about Tocotrienols' anti-angiogenic properties:

Tocotrienols show promise against cancer growth<

"Japanese researchers, led by Kiyotaka Nakagawa from Tohoku University, looked at the ability of tocotrienol to prevent angiogenesis, associated with tumour growth, rheumatoid arthritis, and diabetic retinopathy.

"Our findings suggest that tocotrienol has potential as a therapeutic dietary supplement for preventing angiogenic disorders, and therefore future clinical study will be required to evaluate the efficacy and safety of tocotrienol," wrote the researchers in the Journal of Nutrition."

Here's the actual study:

In Vivo Angiogenesis Is Suppressed by Unsaturated Vitamin E, Tocotrienol<

This study made me wonder how well tocotrienol might help fight the runaway angiogenesis that appears to be involved in rosaceai as well, so I did some digging to find a few topicals that are being formulated with it, some even suggested to help with "spider veins". Interesting. I've included a few of these products here for anyone interested:

Interesting study on Tocotrienols as angiogenesis inhibitors<

I've since run across the above study on Dr Wheldon's site<, where it's been for some time with some additional information, just waiting for me to read again and finally comprehend. The good doctors are always AT LEAST 2 steps ahead of us...

I haven't tried Tocotrienols yet either since I've got my hands full with just the D3 at the moment, but I'm very interested. Please keep us posted on your progress with it. Most of all, best of luck!

On Combined Antibiotic Protocol for Cpni in Rosacea 01/06 - 07/07, On Vit D3 + NACi since 07/07 and daily FIR Sauna since 08/07

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-
Thanks for bringing this to our attention,Jim. When I last spoke with Dr. Powell he also suggested getting some tocotrienol E. As with your experience with Dr. Stratton, I have to ask him to slow down and spell things out for me as I had never heard of this form. I bought some Source Naturals Tocotrienol and have been taking 1 capi a day it for about a month. It says on the label: Vitamin E as D-alpha tocopherol 100mg Total Tocotrienols 50mg yielding gamma-Tocotrienols 24mg As the articles say, it is best to not take tocopherol with tocotrienol. I am wondering about the statement on the label of the supplement I bought that says combining the two enhances E's antioxidant">i properties. So it looks like I will be ordering another brand. Thank you again for setting out this information and clarifying it for us. Now I understand more about the benefits of this form of E. Raven CAP since 8-05 for Cpni and Mycoplasma P. for MSi and or CFSi

Feeling 98% well-going for 100. Very low test for Cpni. CAPi since 8-05 for Cpn/Mycoplasma P.,Lyme, Bartonella, Mold exposure,NACi,BHRT, MethyB12 FIRi Sauna. 1-18-11 begin new treatment plan with naturopath

Raven-

I'm sure you can find more, but this link< says:

As mentioned previously, tocopherols do not have tocotrienols’ cholesteroli-lowering ability. In fact, alpha-tocopherol has been shown to attenuate or interfere with gamma- and delta-tocotrienols’ cholesterol-lowering action.3 Therefore, it is important to find a supplement with a high tocotrienols content (especially gamma- and delta-tocotrienol) and a low tocopherols content. “Rice tocotrienols” contain about 50 percent tocotrienols and 50 percent tocopherols. “Palm tocotrienols” contain approximately 75 percent tocotrienols and 25 percent tocopherols. However, a little known tropical-rainforest-derived plant called Annatto ranks highest in tocotrienol content. Annatto contains 100 percent tocotrienols, and is virtually tocopherol-free. A special patented, solvent-free extraction of Annatto seeds produces the two most effective tocotrienol isomers: 90 percent delta-tocotrienol and 10 percent gamma-tocotrienol. Annatto is the only source of tocotrienols that contains 100 percent desmethyl tocotrienols and virtually no tocopherols.

Annatto-derived tocotrienols should be consumed 6 or more hours away from a multivitamin or other vitamin E supplement, due to the tocotrienol-inhibiting ability of tocopherols (the form used in most multivitamins and vitamin E supplementsi).

 They refer to this study<:

We now report that including alpha-tocopherol in tocol blends containing adequate gamma-tocotrienol to suppress 3-hydroxy-3-methylglutaryl coenzyme A reductase activity results in an attenuation of the tocotrienol action (P less than 0.001)

CAPi for Cpni 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, Cytotec 100mg, Plaquenil 100mg, Magnascent Iodine 12 drps/day, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

 Some other sources (pricey, but I haven't compared dosages to the Nutricology):


http://www.vrp.com/ProductPage.aspx?ProdID=3090<

http://www.healthyheartht.info/deltagold.htm<  

CAPi for Cpni 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, Cytotec 100mg, Plaquenil 100mg, Magnascent Iodine 12 drps/day, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

hmmm, my NOW foods E is only 80 mg of delta, gamma &? mixed so I will look for another brand when this is done!

Thanks very much for the information peeps.

CFIDSi/ME 25yrs, FMSi, IBSi, EBVi, Cpni, (insomnia - melatonin">i, GABA, tarazadone, triazolam, novocyclopine, allergy formula, 3 gm tryptophan), Natural HRT peri-M, NACi 2.5 gm, 6-07 Doxy 200 mg day pm, Azith 375 mg M/W/Fday, 1-3-08 5th pulse 1 X 375 mg 4day

CFIDSi/ME, FMSi, MCS, IBSi, EBVi, CMV, Cpni, H1, chronic insomnia, Chronic Lyme, HME, Babesia, Natural HRT-menopause, NAC 2.4 gm,Full CAP 6-2-07, all supplementsi+Iodorol, Inositol-depression, ultra Chitosan, L lysine Pulse#27 04-19-10 1gm Flagyli/day-5 days<

 Ruth- and the Now Foods is mixed with regular Vit E (tocopherols) which counters the cholesteroli lowering effect of tocotrienol. Unknown whether it also counters the anti-chlamydial nature of it, but the research was done using pure high-delta fraction tocotrienol, so that's what I would use if you try it.

CAPi for Cpni 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, Cytotec 100mg, Plaquenil 100mg, Magnascent Iodine 12 drps/day, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Well this explains why 5 years ago I went on a super food which main claim was to have tocotrienols and I felt TERRIBLE. My blood sugar dropped, weakness etc. Now that I know what I know it was a die off. I always wondered why I did not feel well on it. Thanks for the info. kc dxi msi 1996. started capi 10/05. Started with tinii pulses switched to flagyli pulses. Now almost on continous 500mg flagyl but do need breaks. On doxyi 200mg/day azith 250 mwf and NACi 700mg. ldni 2.0mg.
dxi msi 1996. started capi 10/05. Started with tinii pulses switched to flagyli pulses. Now almost on continous 500mg flagyl but do need breaks. On minoi 100mg/day biaxin 1000mg day and NACi 600mg. ldni 2.0mg.

 kc- I'm not so sure your die off was from toco's. In a communication with the chief scientist on that patent, Dr. Stuart, she did not think that Toco's would cause such reactions since they inhibit entry and infection predominantly. The apoptotic effect appears mostly in cancer cells, and did not show up in Chlamydia in vitro.

It could be that other ingrediants in your "superfood" had an affect: anti-candida die-off; increased porphyrins from effects on the liver; or some other anti-Cpni effect of the ingredients. There is another patent I found which lists plant phenolics and herbal extracts that were found to have antichlamydial effect, especially in combination. A green superfood could have sufficient quantities of these to kick your butt!

We don't yet have any data on people with Cpn load taking tocotrienols ie. starting a high delta formulation and having distinct die-off reactions. Awaiting the reports-- me first! 

CAPi for Cpn 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, Cytotec 100mg, Plaquenil 100mg, Magnascent Iodine 12 drps/day, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Jim and all,  Another lab rat here.  As a matter of coincidence, this weekend I tried to order Allergy Research/Nutricology Delta-Fraction from iherb.com along with my quarterly supplement order, but they were out of stock.  So, I ordered it from another internet supplement vendor and should receive it this week.  On December 11, my total cholesteroli was 311; triglycerides were 159.  On December 28, total cholesterol was 261, and triglycerides were 213.  By the time the samples were collected, I had already been using a good brand of phosphatidyl choline for several weeks (but abusing myself with holiday and comfort food).  The only good news on the lipid front was that the HDL was higher than on previous tests.  Near the end of this month, I will see an endocrinologist (for other reasons) who will probably order new lab work.  It will be interesting to see what the lipid levels are after using the Delta-Fraction for almost 3 weeks.

Incidentally, I wrapped-up the full-time CAPi on January 25 per my doctor's direction.  I think the dates of blood collection were high-inflammationi times related to die-off.

Joyce (self)~generally falling apart with high blood lipids, adult onset diabetes, arthritis, other Cpni and Mpn related conditions, recent trend from hyperostosis to osteoporosis---CAP 5/07-1/08.

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAP since August 06, Cpn, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAPi since August 06, Cpni, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

How long did the continuous CAPi go on for Joyce? Has it made a difference to your recovery?

Michèle (UK) GFAi: Wheldon CAP 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse. Zoo keeper for Ella, RRMSi, At worse EDSSi 9, 3 months later 7 now 5.5 Wheldon CAP 16th March 2006

Michèle (UK) GFAi: Wheldon CAPi 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse.

Jim, what was the plant phenolics and herbal extracts found to have antichlamydial effects? I am continuously flagylating myself since last April. I want something to kick me harder. Combined Antibiotic Protocol minocycline, azithromycin, metronidazolei for muscle pain, insomnia, interstitial cystitisi, sinus, disphonia, dry eyes, stiff neck, veins, thyroid, TMJ.

minocycline, azithromycine, metronidazolei 2007-2009, chelation for lead poisoning, muscle pain, insomnia, interstitial cystitisi (almost well), sinus, dry eyes, stiff neck, veins, hypothyroid, TMJ, hip joints (no longer hurt)

Michele,  It was from May 2007 through January 2008.  I know what you're thinking:  "it's probably not long enough."  I agree, and I don't recommend that short of a run for the typical site user.  In my case, though I believe I have had chronic Cpni infection for a very long time, none of my "conditions" had advanced to a point of serious illness.  My immunei system was still fairly functional when I started the protocol.  At the time I ended the full-time CAPi, I had begun to feel much better all the time without any noticeable die-off reactions for about the last 3 pulses.  I'd like to say I feel great and energetic, but I'm under too much stress that's related to Steve's illness and treatment journey to actually feel very good.  I do have little spurts of energy, though, when I forget about things.  It seems contradictory to say I was not having any noticeable die-off reactions when I said earlier that it was also a time of inflammationi.  What kind of symptoms can you notice from you arteries being cleared?

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAP since August 06, Cpn, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAPi since August 06, Cpni, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

Jim I was on Risotriene which is made from brown rice. There is no green food in it. I definately did have die off from it, I realize that we don't want people to have a pre conceived notion that they will experience die off but everyone is different. This health attempt was WAY BEFORE I did any CAPi. Let's hope no one does experience die off from it. x msi 1996. started cap 10/05. Started with tinii pulses switched to flagyli pulses. Now almost on continous 500mg flagyl but do need breaks. On doxyi 200mg/day azith 250 mwf and NACi 700mg. ldni 2.0mg.
dxi msi 1996. started capi 10/05. Started with tinii pulses switched to flagyli pulses. Now almost on continous 500mg flagyl but do need breaks. On minoi 100mg/day biaxin 1000mg day and NACi 600mg. ldni 2.0mg.

Joyce, I pray this is a good decision for you & Steve is doing better.

CFIDSi/ME 25yrs, FMSi, IBSi, EBVi, Cpni, (insomnia - melatonin">i, GABA, tarazadone, triazolam, novocyclopine, allergy formula, 3 gm tryptophan), Natural HRT peri-M, NACi 2.5 gm, 6-07 Doxy 200 mg day pm, Azith 375 mg M/W/Fday, 1-3-08 5th pulse 1 X 375 mg 4day

CFIDSi/ME, FMSi, MCS, IBSi, EBVi, CMV, Cpni, H1, chronic insomnia, Chronic Lyme, HME, Babesia, Natural HRT-menopause, NAC 2.4 gm,Full CAP 6-2-07, all supplementsi+Iodorol, Inositol-depression, ultra Chitosan, L lysine Pulse#27 04-19-10 1gm Flagyli/day-5 days<

Dr. Stratton is of the opinion that the toco's are probably best used after completing a CAPi as a maintenance and preventative. One reason is their expense compared to other supplementsi. He mentioned that someone like Sarah Longlands, having completed treatment, would benefit from adding tocotrienol as a maintenance supplement along with NACi. The other reason he mentioned is that their main effect appears to be preventing EBi's from invading. This does nothing for Cryptic forms, which he believes can still generate RB's in infected cells, albeit at a slower rate. You still have to get in there and kill it.

But he thought toco's could also be a useful CAP start-up for some who are especially sensitive to the die-off when I mentioned this idea I had had. The start up might be: NAC (until tolerate one per day) then add one tocotrienol a day, continue from there. 

Of course, the effects on cholesteroli alone would make it a worthwhile supplement for many. I'm convinced, as is Dr. Stratton, that cholesterol is ramped up in Cpni infection, either by the bacteria itself upregulating this in the body, or as a defensive response by the body, or perhaps to respond to the organism's hijacking of cholesterol for it's own uses. 

KC- the amount of tocotrienals in Risotriene is pretty small:

Tocotrienols

1.20

mg

http://healthy-living.org/html/risotriene_analysis.html<

There is so much other stuff in it, including plant phenols and sterols, that it's impossible to know what caused your reaction. On the other hand, given your report, it may be a useful supplement that enhances Cpn kill for whatever reason (see patent info below).

 ----------------------------------------------------------------------------------------------------------------------

try http://www.freepatentsonline.com< for a read. 

United States Patent Application
20040058983
Vuorela, Heikki ;   et al.
March 25, 2004

Plant-derived and synthetic phenolic compounds and plant extracts, effective in the treatment and prevention of chlamydial infectionsi

Abstract
The invention relates to natural and synthetic compounds, plant extracts and compositions containing them and mixtures of these in the treatment and/or prevention of a chlamydial infection. Medicinal preparations, food additive compositions and functional foodstuffs can be prepared from the plant-derived phenolic compounds and synthetic compounds and plant extracts.

CAP for Cpn 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, Cytotec 100mg, Plaquenil 100mg, Magnascent Iodine 12 drps/day, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Thanks Ruth,  It was my doctors decision, not mine.  Time will tell what kind of example I turn out to be for the category of people who generally have similar symptoms to a similar degree.  My battling efforts are now focused on newly discovered enemies while my feet are holding down the old ones.

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAPi since August 06, Cpni, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAPi since August 06, Cpni, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

Is this stuff giving anyone else gas and diarrhea, or am I the lucky one? 

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAPi since August 06, Cpni, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

Joyce~caregiver-advocate in Dallas for Steve J (SPMSi).  CAPi since August 06, Cpni, Mpn, B. burgdorferi, systemic candidiasis, EBVi, CMV & other herpes family viral infectionsi, elevated heavy metals, gluten+casein sensitivity. 

 I guess you are just lucky! No problem in this way for me. Curious that you are getting this reaction.

CAPi for Cpni 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, INHi 150mg, Magnascent Iodine 20 drps/day, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Delta Tocotrienol E is now back in stock at iHerb and significantly less than the order I first placed with another online vendor at $14.97.Same brand. Happy supplement hunting--better than those weird colored eggs and alien marshmallow chicks! http://tinyurl.com/yvhuq6< Raven

Feeling 98% well-going for 100. Very low test for Cpni. CAPi since 8-05 for Cpn/Mycoplasma P.,Lyme, Bartonella, Mold exposure,NACi,BHRT, MethyB12 FIRi Sauna. 1-18-11 begin new treatment plan with naturopath

I'm partial to the pastel M&M's, thank you very much, but tocotrienol is right up there on the 'must have' list. I ordered from Iherb and they were out, so I'll try again tonight. Thanks, Raven!

The difference between what we do and what we are capable of doing would suffice to solve most of the world’s problems.  Mohandas Gandhi

The difference between what we do and what we are capable of doing would suffice to solve most of the world’s problems. Mohandas Gandhi

Thanks, I will check IHerb as well!

I love the Cadbury mini eggs with that lovely candy coating & now, even in dark chocolate!

Happy Easter everyone!

CFIDSi/ME 25yrs, FMSi, IBSi, EBVi, Cpni, (insomnia - melatonin">i, GABA, tarazadone, triazolam, novocycloprine, allergy formula, 3 gm tryptophan), Natural HRT peri-M, NACi 2.5 gm, 6-07 Doxy 200 mg day pm, Azith 375 mg M/W/Fday, 8th Pulse 2 X 375 mg 3day,375 2 d

CFIDSi/ME, FMSi, MCS, IBSi, EBVi, CMV, Cpni, H1, chronic insomnia, Chronic Lyme, HME, Babesia, Natural HRT-menopause, NAC 2.4 gm,Full CAP 6-2-07, all supplementsi+Iodorol, Inositol-depression, ultra Chitosan, L lysine Pulse#27 04-19-10 1gm Flagyli/day-5 days<

 I've had good service and low price from www.outletnutrition.com< including good pricing on Now Calcium Pyruvate. Just ordered a bunch as the latter was on sale!

CAPi for Cpni 11/04. Dxi: 25yrs CFSi & FMSi. Protocol: 200mg Doxyi, 500mg MWF Azith, Tinii 1000mg/day pulses; Vit D1000 units, Iodoral 50mg, T4 & T3

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Last week, or perhaps the week before, I posted something that mentioned that I was having trouble finding a good source of Tocotrienols.  Jim K replied with a link to a site that has them from Nutricology.

So, I went out looking for all sites that carry Nutricology tocotrienols and what did I find but a link to this discussion albeit, it was about 2/3rds of the way down the page.  However, it was there.

Now, my reasons for looking for the link is I'm about 28 days away from needing to refill my initial 2 month supply of tocotrienols, which I got from Vitamin Research Products.  Jim's source from Nutricology was much lower priced and I'll go that way in the future.

As mentioned, I've been on Annatto tocotrienols for over a month.  I do notice a reaction from them, generally about 15 minutes after taking one pill of 100mg.  It's what would be described as mild in comparison to some reactions that people here experience, but it is a reaction.  Basically, it makes my limbs heavier and movement more lethargic, your basic herx type reaction for someone with MSi, or so I understand.

I also usually take the tocotrienols within 45 - 60 minutes of taking a dose of Rifampin, NACi, and INHi

all my best

John

RRMSi/EDSSi was 4.5, was 4.???, now 5 on Wheldon Protocol (nac, doxycycline, azithromycin, metronidazolei) since 04/12/2006. Added Rifampin 2x150mg/daily on 08/19/2007. Added INH 300mg/daily on 03/17/2008.

best, John

RRMSi/EDSSi was 4.5, 5, 6, 6.5, 6.9999, 6.5 on Wheldon/Stratton Protocol beginning 04/12/2006
naci 4x600 mg/day
doxycycline 2x100mg/day
azithromycin 3x250mg/day MWF
metronidazolei 3x400mg/day then 3x500mg/day

Annatto tocotrienols are back in stock at Iherb.com too.

Michèle (UK) GFAi: Wheldon CAPi 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse. Zoo keeper for Ella, RRMSi, At worse EDSSi 9, 3 months later 7 now 5.5 Wheldon CAP 16th March 2006

Michèle (UK) GFAi: Wheldon CAPi 1st May 2006. Daily Doxyi, Azi MWF, metroi pulse.

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