Repeated Chlamydia pneumonia infection, persistence, caridovascular disease, luteolin

I don't believe we have this linked to our Research Pages (Marie?). This is a brilliant dissertation from the Finnish group, some of the world's experts on Cpni as some of the faculty in Helsinki were part of the original group who discovered the very existence of Cpn.

This dissertation demonstrates a number of important findings:

  • Repeated infection with Cpn "...induced persistent chlamydial DNA and inflammationi in lung tissue and development of mouse Hsp60 autoantibodies."
  • Repeated infection with Cpn "...significantly increased subendothelial lipid accumulation in the aortic sinus area."
  • That "A flavonoid, luteolin, was shown to effectively decrease the chlamydial load and inflammatory reactions in lung tissue." Note: luteolin is not the same as lutein.
  • Conventional antimicrobial treatments are not effectively to eradicate persistent infection.
Go to the link and you can download the whole thing in pdf form.
Experimental Chlamydia pneumoniae infection model: effects of repeated inoculations and treatment

Liisa Törmäkangas

Lääketieteellinen tiedekunta, Oulun yliopisto

Mikrobiologian laitos, Oulun yliopisto

Kansanterveyslaitos, -

Academic Dissertation to be presented with the assent of the Faculty of Medicine, University of Oulu, for public discussion in the Auditorium of Kastelli Research Center (Aapistie 1), on January 21st, 2006, at 10 a.m.

Copyright © 2005

Oulun yliopisto

Esitarkastajat

Professor Ignatius W. Fong

Docent Kimmo Mattila

OULUN YLIOPISTO, OULU 2005

ISBN 951-42-7976-X

URN:ISBN:951427976X

Abstract

Chlamydia pneumoniae is a common human pathogen worldwide, which causes both upper and lower respiratory tract infectionsi. In addition, C. pneumoniae infections have been associated with atherosclerosis and other chronic diseasesi, and successful treatment and eradication of the organism from tissues would therefore be desirable. The purpose of this study was to assess the effects of C. pneumoniae inoculations on the development of chronic infection and atherosclerotic changes in normocholesterolemic, wild-type mice. We also aimed to elucidate the effects of antibiotic and other treatments on the eradication of chlamydia and on the reduction of the pathologic sequelae induced by these infections.

Female C57BL/6J mice were fed either normal chow when assessing the effects of acute infection, or a diet supplemented with 0.2% cholesterol when evaluating the atherosclerotic changes. Primary or repeated inoculations with C. pneumoniae isolate K7 were given to the mice intranasally, and the effects of treatments with telithromycin, levofloxacin and erythromycin antimicrobial agents and with the phenolic compounds quercetin, luteolin and octyl gallate were evaluated. The following methods were used to measure infection and treatment effects and the presence of chlamydia in tissue: chlamydia culture, PCRi and RT-PCR methods, histology of lung, heart and aortic tissue, serologic methods and measurements of aortic contractility responses.

Repeated C. pneumoniae inoculations induced persistent chlamydial DNA and inflammation in lung tissue and development of mouse Hsp60 autoantibodies. Infection was shown to influence aortic endothelial function, and repeated inoculations significantly increased subendothelial lipid accumulation in the aortic sinus area. A flavonoid, luteolin, was shown to effectively decrease the chlamydial load and inflammatory reactions in lung tissue. All antimicrobial agents eradicated the presence of viable chlamydia effectively; however, PCR positivity persisted in lung tissue despite the treatments. Only immediate treatment after each inoculation was able to decrease aortic sinus lipid accumulation.

In conclusion, these data support the role of C. pneumoniae in promoting atherosclerotic development via autoimmune responses and also via direct effects on aortic tissue. Conventional antimicrobial treatments may not effectively eradicate persistent infection, and further studies are warranted to seek for alternative treatment options.

Asiasanat: antibacterial agents, aorta, atherosclerosis, C57BL mice, Chlamydia pneumoniae, chronic disease, flavonoids, persistent infection, pneumonia, vascular endothelium

http://herkules.oulu.fi/isbn951427976X/<

Comments

Hi Jim,

Funny, I just read about luteolin last week and ran across this study and some others that seem interesting:

 

Luteolin & nuclear factor-kappa B<

Luteolin & interferon gamma<

Luteolin & chlamydia<

 

Anti-inflammatory effects and anti-chlamydia effects? Hmmm...

At the risk of you telling me again to stop experimenting, I couldn't resist.   I have already ordered some supplementsi - Lutimax - couldn't find much on this supplement other than some comments on ALS boards where people tried it apparently unsuccessfully (and sadly).   Hopefully they'll show up in the next day or two.   Will let you know what I find...

By the way, I tried Bromelain alone for a week (without quercetin), and noticed it seemed to cause symptoms to decrease while on it (actually quite a bit - it may have helped with porphyriai), but when I stopped I may have noticed a bit of an increase from prior to taking it.   So be careful with it... 

Red 

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-

 Hi Red- Bromelain is anti-inflammatory by itself, breaks down inflammatory proteins. The luteolin stuff is very significant, but the dosing issue is quite the question. Some things like quercetin show in some studies to be antichlamydial and others to be prochlamydial-- it may be that above a certain level it is prochlamydial. So... don't max the Luteamax! Try at recommended dose levels first, and then vary up and down to find the one which has effect.

Combined Antibiotic Protocol for Chlamydia pneumonia in Chronic Fatigue Syndromei &amp; Fibromyalgiai- Currently: 150mg INHi, Doxycycline/Zithromycin, Tinidazole pulses. Northern Ohio, USA

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Luteolin is found in artichokes, which Sarah and I both love. Giorgio de Chirico liked them, too; they feature mysteriously in many of his paintings. (See http://en.wikipedia.org/wiki/Image:Gare_Montparnasse.jpg< )

Here's an interesting abstract on luteolin and vasomotion in aged rats:

Rossoni G, Grande S, Galli C, Visioli F.

Wild artichoke prevents the age-associated loss of vasomotori function.

J Agric Food Chem. 2005 Dec 28;53(26):10291-6.

'. . . . the feeding of wild artichoke [10 mg (kg of polyphenols)(-1) day(-1)] to aged rats significantly restores proper vasomotion, to a degree similar to that observed in young animals. This study provides further justification to the advice to consume wild greens as part of a balanced diet and suggests that close attention should be paid to the diet of the elderly, because it can effectively modulate important parameters of cardiovascular risk.'

D W - [Myalgia and hypertension">i (typically 155/95.) Began (2003) taking doxycycline and macrolide and later adding metronidazolei. No medication now. Morning BP typically 110/75]

 

Thanks David.   And it also appears that you can purchase supplementsi with extract of artichoke leaves, containing luteolin, but I was afraid they might also contain quercetins or salicylates in general, and I worried this might counteract the effects of the luteolin (particularly since I seem to be so sensitive to salicylates):

Phenolic compunds in artichoke waste <

BTW, I also love artichokes, particularly dipped in blue cheese dressing.   Of course almost anything would be good this way in my book, and I'm sure it was only by accident that blue cheese dressing was left out as the 6th Basic Sauce in all those Chef books (along with the 7th Basic Sauce - melted Velveeta, aka Brie American!).   Sorry, it's time for lunch and I'm a little hungry...

 
Also thanks for the warning Jim.   I'm a little leery of Lutimax anyway since I couldn't find much on it but I thought I'd give it a tentative try.   Since studies show that luteolin and quercetins / salicylates seem to work through similar mechanisms (inhibiting NF-KappaB) and all seem to inhibit the cox pathway(s), I'm also a little leery that luteolin might be similar to aspirin for me - too much "pain" for the gain.   Still, it would be nice if it works differently enough to afford gain without pain.   It appears to have some slight differences (note differing effects on iNos and eNos):

Quercetin vs Luteolin on Cpn< 

 

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-

Jim, just received the Lutimax supplementsi and also just found this study on luteolin for leishmania which suggests a dosage of 3.5mg / kg is effective (for that parasite at least).   Note also their conclusions on quercetin dosage:

Luteolin & leishmania< 

The dosing on the Lutimax bottle suggests taking 1 100mg tablet 4 x a day.    This seems a bit high for my weight per my calculations compared to the above study.   Per my calcs, 3.5mg / kg for my 165 lbs is @ 262.5mg.   So, I think I'll start with 1 100mg tablet 2 x a day and see how it goes...  

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-

Interesting posts about luteolin. i just came back from seeing Dr. Powell and he has put it on his list of Inflammatory Pathway Inhibitors. Also on the list: Chlorella, Curcumin, Indole-3-Carbinol, Nicinamide (and also Boron). The overall champ at reducing inflammatory agents came out to be curcumin (tumeric) at 900 to 1200 mgs a day).

Raven 

Feeling 98% well-going for 100. Very low test for Cpni. CAPi since 8-05 for Cpn/Mycoplasma P.,Lyme, Bartonella, Mold exposure,NACi,BHRT, MethyB12 FIRi Sauna. 1-18-11 begin new treatment plan with naturopath

Thanks Raven.   Glad to hear Dr Powell actually has luteolin on his list.  

Interesting about Curcumin.   I couldn't find too much relating on it relating to Chlamydia, but I did find the following:

Curcumin & Chlamydia <

In addition to being a dual cox & 5-lox inhibitor, it also seems to inhibit NF-KappaB like luteolin and quercetins:

Curcumin & NF-KappaB<

I tried Curcumin (can't remember the dosage) before going on the abxi and had a pretty violent reaction to it (flushing, congestion, rashes) as I did with quercetin and other dual cox/5-lox inhibitors and cox inhibitors (aspirin in particular).   It could be their anti-chlamydial activities that seem to increase symptoms for me.   Or it could be just the way I unfortunately react to cox inhibitors.   Interestingly though after being on abxi for several months now, I don't seem to react so violently to salicylates, so Curcumin might be worth a try again to see if I get some benefit without too much "pain".   

I'll first see how I react to luteolin...

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-

Hi Red, Give it a try. You could start with a small amount. He gave me a handout that iists 41 different inflammatory mediators with a special emphasis on IL-8 (tumor growth) that are affected (lowered) by curcumin. I told him I found organic turmeric root at a local farmstand and have been pressing it in a garlic press into tomato juice. The capsules should be OK too.

It has seemed to make a difference for me. I didn't take it yesterday as I was traveling and felt worse today. 

He says the chlorella is very effective too.

Raven 

Feeling 98% well-going for 100. Very low test for Cpni. CAPi since 8-05 for Cpn/Mycoplasma P.,Lyme, Bartonella, Mold exposure,NACi,BHRT, MethyB12 FIRi Sauna. 1-18-11 begin new treatment plan with naturopath

Dr. P had me adding luteolin by taking a double dose of Entrox by Now Vitaminsi, an Omega-3 supplement from perilla seed oil (a source of luteolin). He seems more confident of the dosing now of the Lutimax, though, so I'll use this up and try that eventually.

Jury is still out on Curcumin for me as I'm still doing Tinidazole, and don't have a reference point yet! 

Combined Antibiotic Protocol for Chlamydia pneumonia in Chronic Fatigue Syndromei &amp; Fibromyalgiai- Currently: 150mg INHi, Doxycycline/Zithromycin, Tinidazole pulses. Northern Ohio, USA

 

CAPi for Cpni 11/04. Dxi: 25+yrs CFSi & FMSi. Currently: 250 aithromycin mwf, doxycycline 100mg BIDi, restarted Tinii pulses; Vit D2000 units, T4 & T3, 6mg Iodoral

Glad to hear Dr P seems confident in Lutimax.   Makes me feel a little better about it.   I took my initial dose @ 5 hours ago and no initial negative side-effects so far.   Flushing, congestion, etc often show up for me within the first couple of hours or, if not then, after 2 or 3 days.   I'll let you know as soon as recognize any positive or negative effects.

Keep us posted on your results with curcumin too, if you both don't mind.   Good luck with it...
    

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-

A couple of other interesting articles on luteolin:

Luteolin & LPS-induced Lethal Toxicity <

Luteolin & Experimental Model of MS <

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-

Hi All, I've been taking the luteolin in the form of Lutimax lozenges,  which contains 100 mg luteolin (along with 10 mg Vit C, 50 IU Vit D, 5 mg Vit E, 50 mg calcium and 100 mg rutin), twice a day for a week now and it seems to be helping quite a bit with symptoms.  

The remaining rash on my stomach has cleared up nicely, I have even noticeably less edema in my face, neck and elsewhere, and it seems to have helped clear up the cough that reappeared immediately after stopping the flagyli dose I was taking twice a week (I've decided to go to the standard 5 days a month pulse).   It also has seemed to help give me energy (or rather decrease the fatigue), help with some remaining IBSi symptoms, and help eliminate most of the aches and pains I seemed to experience even while off the flagyl.  

I did have a glass and a half of wine the other night and noticed that I ended up tossing and turning all night and woke up in a pool of sweat a couple of times, and woke up with a bit of a rash that cleared up quickly, so I don't think I'll be drinking while on it.

All in all, it seems to help pretty well with symptoms and this kind of makes me a bit nervous based on my experience before with things (5-lox inhibitors) that masked symptoms well but seemed to allow these little buggers to multiply underneath the mask.   I think I'll tentatively continue using it as it seems to provide benefit while studies seem to suggest it kills Cpni, although I have no idea if it is actually killing any Cpn.   

I will also report its interaction with flagyl on my next pulse (I'm due for one, but I'm kind of enjoying being pretty much symptom free right now.)  I'm also hoping it helps with the endotoxini die-off reaction based on some of the studies above, but we'll have to see...

 

Treatment for Rosaceai<

  • CAPi:  01/06-07/07
  • High-Dose Vit D3, NACi:  07/07-11/08
  • Intermtnt CAP, HDose Vit D3:  11/08-01/09
  • HDose Vit D3, Mg, Zn: 01/09-

I was sure we had it already but I ended up adding it to the cardiovascular pages...thanks!
marie
On CAPi since Sept '05 for MSi, RAi, Asthmai, sciatica. EDSSi at start 5.5.
"Color out side the lines!"

On CAPi since Sept '05 for MSi, RAi, Asthmai, sciatica. EDSSi at start 5.5.(early cane) Now 6 (cane full time) Originally on: Doxyi 200, Azith 3x week, Tinii cont. over summer '07, Revamp of protocol in Summer '08 by Stratton due to functional loss; clarithro

I did not read the whole paper but was excited when I found it.  Glad to see that everyone else here got it posted long ago.

I wonder, what came of this - in terms of supplementsi.  I've not noted that folks include Luteolin on theie sups lists.  Or is it duplicative of other sups we all take like chlorella or curcumin?  As I scanned through I noticed a lot of common terminology with MS, hummmm...

On a side note, I found this while in search of a journal article that explains the 3 phases of CPni.  Most only acknowledge EBi and RB, but not Cryptic.  Ken

In pursuit of ABX<

Don't Allow What You Know To Get In The Way Of What Might Be

I knew I would find something about Luteolin here! 

Come new warrers, see this topic too

Very interesting reading.  I've been on Swanson's Luteolin complex 100 mg now for about 6 weeks since I read an article about it.  The only difference I notice is that I constantly have a loose cough.  Irene

That cough may be a result Of endotoxini I suppose and hope so!

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