Alzheimers and Chlamydia Pneumoniae

CPn infection promotes transmigration of monocytes through human brain endothelial cells [1]

Chlamydophyla (chlamydia)pneumoniae in the AD brain [2] More work by Brian Balin. Once again we see very good evidence of CPn being a neural pathogen.

Chlamydia pneumoniae in the alzheimers brain [3] This work is on AD but the authors discovered CPn in the cells of the brain near the AD plaques. This should help establish CPn as a potentially neural pathogen

-Chlamydia pneumoniae in the Alzheimer's brain varies with APOE genotype [4] The APOE genotype apparently interacts with CPn in alzheimers patients carrying that genotype. This suggests an interesting theory: that a bacteria is not the same in every person in terms of effects but rather an interaction between genesi [5] and bugs results in the pathology an individual experiences. This is a whole new understanding of how we interact with our environment.

The Balin research related to CPn in Alzheimer's Disease [6] This list of research abstracts with links is an important study for anyone interested in CPn in the brain. Of note, this researcher like Dr Sriram also finds CPn in the brain consistently when others do not. This is likely due to the fact that both VU and Balin use frozen, not formalin fixed, tissue.

High prevalence of CPn antibodies in vascular dementia [7] While VD is not alzheimers it is another indication that CPn has an affinity for cerebral tissue and is related to loss of brain integrity

Chlamydophila pneumoniae cultured from the late-onset Alzheimer brain

Int J Med Microbiol. 2008 Sep 29. [Epub ahead of print]Click here to read

Initial characterization of Chlamydophila (Chlamydia) pneumoniae cultured from the late-onset Alzheimer brain.

Dreses-Werringloer U, Bhuiyan M, Zhao Y, Gérard HC, Whittum-Hudson JA, Hudson AP. Department of Immunology and Microbiology, Wayne State University School of Medicine, Gordon H. Scott Hall, 540 East Canfield Avenue, Detroit, MI 48201, USA.

Previous studies from this laboratory provided evidence that the intracellulari [8] bacterial pathogen Chlamydophila (Chlamydia) pneumoniae is present in the late-onset Alzheimer's diseasei [9] (AD) brain. Here we report culture of the organism from two AD brain samples, each of which originated from a different geographic region of North America. Culturable organisms were detectable after one and two passages in HEp-2 cells for the two samples. Both isolates, designated Tor-1 and Phi-1, were demonstrated to be authentic C. pneumoniae using PCRi [10] assays targeting the C. pneumoniae-specific genesi [5] Cpn0695, Cpn1046, and tyrP. Assessment of inclusion morphology and quantitation of infectious yields in epithelial (HEp-2), astrocytic (U-87 MG), and microglial (CHME-5) cell lines demonstrated an active, rather than a persistent, growth phenotype for both isolates in all host cell types. Sequencing of the omp1 gene from each isolate, and directly from DNA prepared from several additional AD brain tissue samples PCR-positive for C. pneumoniae, revealed genetically diverse chlamydial populations. Both brain isolates carry several copies of the tyrP gene, a triple copy in Tor-1, and predominantly a triple copy in Phi-1 with a minor population component having a double copy. This observation indicated that the brain isolates are more closely related to respiratory than to vascular/atheroma strains of C. pneumoniae.

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CAPi [11]i [11] for Cpni [12] 11/04. Dx: 25yrs CFSi [13]i [13] & FMSi [14]i [14]. Currently: 150mg BID Roxithromycin, Doxycycline 100mg BID, Tinii [15]i [15] 1000mg/day pulses; Vit D2000 units, T4 & T3