Neurosci Res. 2008 May 20.

Chlamydophila pneumoniae DNA and mRNA transcript levels in peripheral blood mononuclear cells and cerebrospinal fluid of patients with multiple sclerosis.

Contini C, Seraceni S, Castellazzi M, Granieri E, Fainardi E. Section of Infectious Diseasesi [1], Department of Clinical and Experimental Medicine, University of Ferrara, via Fossato di Mortara 23, 44100 Ferrara, Italy.

Chlamydophila pneumoniae DNA and mRNA transcripts were investigated by PCRi [2] and RT-PCR in fresh CSF and PBMC specimens co-cultured in Hep-2 cell lines and collected from 14 patients with definite RR MSi [3] and 19 patients with other inflammatory (OIND) and non-inflammatory (NIND) neurological controls. A positivity for C. pneumoniae DNA and mRNA was detected in CSF and PBMCs of 9 RR MS patients (64.2%) with evidence of disease activity, whereas only 3 controls were positive for Chlamydial DNA. These preliminary findings suggest that C. pneumoniae may occur in a persistent and metabolically active state at both peripheral and intrathecali [4] levels in MS, but not in OIND and NIND.

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CAPi [5]i [5] for Cpni [6]i [6] 11/04. Dx: 25yrs CFSi [7]i [7] & FMSi [8]i [8]. Currently: 150mg BID Roxithromycin, Doxycycline 100mg BID, Tinii [9] 1000mg/day pulses; Vit D2000 units, T4 & T3