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Glucocorticoids Increase In Vitro and In Vivo Activities of Antibiotics
By Jim K
Created 05/24/2008 - 11:30pm

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Dec. 2004, p. 4878-4881 Vol. 48, No. 12 0066-4804/04/$08.00__0 DOI: 10.1128/AAC.48.12.4878-4881.2004 Copyright © 2004, American Society for Microbiology. All Rights Reserved.   Glucocorticoids Increase In Vitro and In Vivo Activities of Antibioticsi [1] against Chlamydophila pneumoniae   Dario Caronzolo, Valeria Lucini, Marilou Pannacci, Silvia Grosso, Federica Colleoni, Franco Fraschini, and Francesco Scaglione* Department of Pharmacology, Chemotherapy, and Toxicology, University of Milan, Milan, Italy The in vitro and in vivo antichlamydial activities of dexamethasone and beclomethasone alone and in combination with an antibiotic were tested. In vitro, dexamethasone and beclomethasone decreased the number of inclusion-forming units versus the control number (P < 0.001). the combination of glucocorticoids with azithromycin, telithromycin, or levofloxacin was more active than antibiotics used alone (p < 0.001). the combination, tested in a murine chlamydophila pneumoniae infection model, produced similar results.   They go on to conclude (excerpt):   The previous in vivo studies showing that cortisone reactivates C. pneumoniae in vivo via an immunosuppressant effect (13) are not in disagreement with our data. We used an in vivo dose of Dex and Beclo (120 __g/kg/day) significantly lower than the cortisone acetate dose tested in the past (125 mg/kg/day). Our dose of Dex might mainly lead to an anti- inflammatory effect, while the dose of cortisone leads to an immunosuppressant effect. Moreover, cortisone acetate was used in infected mice carrying a latent infection while we treated a primary infection. Our data are indirectly in agreement with a recent study in which, in subjects with severe asthma, anti-inflammatory doses of steroids were found to be associated with lower titers of immunoglobulin G and A antichlamydial antibodies... ...In a primary infection, an anti-inflammatory and/or a light immunosuppressant effect might be required to counteract the evolution of C. pneumoniae in latent or persistent forms, keep- ing C. pneumoniae inclusions in an antibiotic-susceptible form. ___________________________________________________________   CAPi [2] for Cpni [3] 11/04. Dx: 25yrs CFSi [4] & FMSi [5]i [5]. Currently: 150mg BID Roxithromycini [6], Doxycyclinei [7] 100mg BID, Tinii [8] 1000mg/day pulses; Vit D2000 units, T4 & T3

ANTIMICROBIAL AGENTS AND

Submitted by Jim K on Sat, 2008-05-24 23:43.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Dec. 2004, p. 4878–4881 Vol. 48, No. 12 0066-4804/04/$08.00

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CAPi [2] for Cpni [3] 11/04. Dx: 25yrs CFSi [4] & FMSi [5]. Currently: 150mg BID Roxithromycin, Doxycycline 100mg BID, Tinii [8] 1000mg/day pulses; Vit D2000 units, T4 & T3

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    ANTIMICROBIAL AGENTS [9]

Submitted by Jim K on Sat, 2008-05-24 23:44.
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Dec. 2004, p. 4878–4881 Vol. 48, No. 12  0066-4804/04/$08.00

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CAPi [2] for Cpni [3] 11/04. Dx: 25yrs CFSi [4] & FMSi [5]. Currently: 150mg BID Roxithromycin, Doxycycline 100mg BID, Tinii [8] 1000mg/day pulses; Vit D2000 units, T4 & T3

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Jim, interesting post!  i [10]

Submitted by padeyas on Sun, 2008-05-25 00:22.

Jim,

interesting post!  i have tried MANY CFS meds/supplementsi [11].  To  DATE, low dose (<25mg) hydrocortisone (cortef) has helped me more then anything..  my old physician (Holtorf) just completed a study http://www.hormoneandlongevitycenter.com/nss-folder/pictures/JOURNAL_CFSF.pdf [12] and  "Safe Uses of Hydrocortisone" by William Jefferies MD is a good read for any chronic fatigue/Fibro patient.  we must realize HIGH dosages of prednisone, prednisolone, etc (what is commonly prescribed in health care) CAN be very dangerous (immunei [13] suppresive), but low dose hydrocortisone can actually have a paradoxal effect (boost immune system).  keep in mind that 5mg of predisone is equivalent to 1.25mg of hydrocortison (cortef).

i was initially resistant when i was asked to try the script.  For years, i have thought this catabolic hormone was evil!  You cant pick up a health magazine or watch some informertial and NOT hear how lowering your cortisol can turn you into some supermodel.  well, first lets keep in mind that MIGHT be true for some of the general public (NOT CFS/fibro) patients.  second, high doses of cortisol can be detrimental to the body, but LOW DOSE (<25mg) hydrocortisone is NOT equivalent to excessive cortisol.  there is much debate whether low dose can suppress our own negative feedback system and because there have been some days (not many) that i have not taken any cortef with NO adrenal crash i am convinced it does NOT suppress ones own system.  

well, i am just ECSTATIC to hear its anti-Cpn effects...

Keith

Doxyi [7] 100mg q12hr; Biaxin 500mg q12hr; Amoxicillini [14] 500mg q12hr;  Armour 1 grain; T3;  Cortef 15mg; 4000 units Vitamin Di [15]; Ursodiol; prescription enzymes; life extension vitamin/minerals

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Doxyi [7] 100mg q12hr; Biaxin 500mg q12hr; Amoxicillini [14] 500mg q12hr;  Armour 1 grain; T3;  Cortef 15mg; 4000 units Vitamin Di [15]; Ursodiol; prescription enzymes; life extension vitamin/minerals

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>keep in mind that 5mg of

Submitted by NellyP on Sun, 2008-05-25 05:47.

>keep in mind that 5mg of predisone is equivalent to 1.25mg of >hydrocortison (cortef).

Keith,

I don't want to be pedantic but are you sure of the figures you are quoting?

Nelly (France-neuroLyme and ????)

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Nelly (France-neuroLyme and ????)

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Jim, Sorry, but I can't [16]

Submitted by NellyP on Sun, 2008-05-25 06:09.
Jim, Sorry, but I can't find the article you are quoting. Have you posted it some place? An abstract? I am very interested in this Nelly (France-neuroLyme and ????)

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Nelly (France-neuroLyme and ????)

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Good article Jim. Dr.

Submitted by lee on Sun, 2008-05-25 06:13.
Good article Jim. Dr. Thomas Mcpherson Brown used aspirin or low dose steriods along with antibioticsi [1]. His theory was that mycoplasmas clocked themselves in inflammationi [17]. For the antibiotics to get to the target you had to get the imflammation down. Makes sense that steroids alone activates cpn but given with antibiotics it could be beneficial. Not that I want to try this. The last time I took prednisone it landed me in the hospital with a huge flare. I was not on antibiotics at the time. zero negative sjogren's diagnosed 2/03, 200mg doryx daily, mwf zithromax, flagyli [18] every 3 weeks.

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zero negative sjogren's diagnosed 2/03, 200mg minocin daily, biaxin,plaquinel mwf, flagyli [18] every 3 weeks.

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Nelly try this link

Submitted by garcia on Sun, 2008-05-25 06:29.

Nelly try this link here:

Glucocorticoids Increase In Vitro and In Vivo Activities of Antibiotics [19]

 

CFS. Started CAP 03-07. Currently: Roxi 600mg + Doxyi [7] 200mg . Tinii [8] pulses 1000mg. Sauna QOD. D 8000IU. Niacini [20] 3 x 500mg. Mel 3mg.

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Hunter: Don't think - experiment

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Thanks for the link Garcia-

Submitted by Jim K on Sun, 2008-05-25 07:13.

Thanks for the link Garcia- I tried five times to post the whole thing including link, abstract and conclusions but the damned thing wouldn't take! Don't know why. Didn't like the formating or something. I'll try again. 

CAP for Cpn 11/04. Dxi [21]: 25yrs CFS & FMSi [5]. Protocol: 200mg Doxyi [7], 300mg Roxithromycin, Tinii [8] 1000mg/day pulses; Vit D1000 units, Iodoral 50mg, T4 & T3

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CAPi [2] for Cpni [3] 11/04. Dx: 25yrs CFSi [4] & FMSi [5]. Currently: 150mg BID Roxithromycin, Doxycycline 100mg BID, Tinii [8] 1000mg/day pulses; Vit D2000 units, T4 & T3

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Their mice were killed

Submitted by lifeontheice on Sun, 2008-05-25 08:20.
Their mice were killed after 5 days of treatment on the 9th day of infection. The results might not apply to us at all. 5mg of prednisone is an equivalent to 20 mg of hydrocortisone Dexamethasone 120 micrograms/kg/24hr is a fairly high dose. Usual pediatric doses are 30-200 micrograms/kg/24hr, 250 micrograms/kg/24hr are used in brain edema. Barbara Used to have multiple sclerosis, on the intermittent Wheldon protocol since March 2006, EDSS 0 for over 4 years

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Cured of multiple sclerosisi [22], stopped the Wheldon's protocol in Nov,2008. EDSSi [23] 0 for over 5 years.

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Thanks for posting

Submitted by jeanneroz on Sun, 2008-05-25 09:39.

Thanks for posting this info and link!    I started Cortef about 2 months ago and was concerned because the info that came with it stated it can "lower your ability to fight infection"  I, too, was hesitant to take it (I was using Isocort)

I am on Armour, 2 grains (I have no thyroid and haven't for 10 years), and  also take T3 occasionally.  I was hoping Cortef would give me the energy boost I needed... but so far...... still the same.  But hey, if it's helping  fight the CPN, great!

Jeanneroz ~CPN diagnosed and started protocol  4/2007, also have  HHV6, EBV.  CFIDS/FM diagnosed: 6/07; Protocol:  200 mg/doxyi [7] daily~ 250 mg AZITH M/W/F ~1st Tindi pulse 4/17/08- 1 250 mg. tab for 2 days. supplementsi [11]

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JeanneRoz~CPNi [3] diagnosed & started protocol 4/2007, also HHV6, EBVi [24]. CFIDSi [25]/FM diagnosed: 6/07; 100mg/doxyi [7]/BID ~ 250 mg AZITH M/W/F ~1st Tinii [8] pulse 4/17/08- 1 250 mg. tab for 2 days. Pulse 5: 9/28/08, 250 mg TINI BID, 3 days. Sup

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Thanks for the link

Submitted by NellyP on Sun, 2008-05-25 11:18.
Thanks for the link Garcia. They seem to be saying that adding glucocorticoids to abxi [1] will only enhance the effect of abx if they are given at sub MIC levels, they say that when abx were given at MIC then the results were similar to corticoids + abx. So in fact one has the choice: abx at sub MIC + corticoids or abx at MIC. quotes from the article: "In addition, the combination of AZM (at one-half of the MIC) and Dex, which was significantly more active than the antibiotic alone (P  0.001), produced effects similar to those of AZM at the MIC, reducing the antibiotic concentration necessary to satisfy the requirements of the MIC definition (15)." "Corticosteroids evidently potentiate antibiotic activity when the latter are used at sub-MICs. At the MICs of the antibiotics, the combination produced results similar to those obtained with the antibiotics alone." Shades of Trevor Marshall? Nelly (France-neuroLyme and ????)

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Nelly (France-neuroLyme and ????)

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oops, sorry about

Submitted by padeyas on Sun, 2008-05-25 11:35.

oops, sorry about that, "lifeontheice" has it correct.  5mg of prednisone has 20mg of hydrocortisone in it... 

 

Doxyi [7] 100mg q12hr; Biaxin 500mg q12hr; Amoxicillini [14] 500mg q12hr;  Armour 1 grain; T3;  Cortef 15mg; 4000 units Vitamin Di [15]; Ursodiol; prescription enzymes; life extension vitamin/minerals

___________________________________________________________
Doxyi [7] 100mg q12hr; Biaxin 500mg q12hr; Amoxicillini [14] 500mg q12hr;  Armour 1 grain; T3;  Cortef 15mg; 4000 units Vitamin Di [15]; Ursodiol; prescription enzymes; life extension vitamin/minerals

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Source URL (retrieved on 12/01/2008 - 2:03pm): http://www.cpnhelp.org/glucocorticoids_increase_

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[8] http://www.cpnhelp.org/chlamydia_pneumoniae/an_0
[9] http://www.cpnhelp.org/print/4483#comment-33516
[10] http://www.cpnhelp.org/print/4483#comment-33517
[11] http://www.cpnhelp.org/taxonomy/term/63
[12] http://www.hormoneandlongevitycenter.com/nss-folder/pictures/JOURNAL_CFSF.pdf
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[18] http://www.cpnhelp.org/taxonomy/term/44
[19] http://aac.asm.org/cgi/reprint/48/12/4878.pdf
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