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Finding CPn - Dark Field Microscope?
By Mark Hall
Created 04/20/2008 - 4:59pm

  • Cpn-related research: Member-posted

I hope Jean UK doesn't mind, but I will quote a recent post by her:-

"What I dont understand is if these bugs can be so easily found under a dark field microscope why aren't other doctors looking for and finding them."

I have wanted to ask this question many times over the past few weeks, but kept forgetting.

Paula is also a patient of Dr AW in the UK.  He found her CPni [1] using this method of detection.  I understand that you have to have a very good eye for detecting bacteria using this method.

What do you all think?

Is this a good way to look for CPn, saying as PCRi [2] tests are pretty inacurate?

Mark

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UK Carer of bedridden Severe CFS Feb06. Tick bites Summer04.  CPNi [1] dx.Apr07. Borrelia dx Sept08. Samento 15 drps daily July07.  200mg Doxyi [3]i [3] Jan08.  300mg Roxy Apr08 Stopped abxi [4]i [4] Nov/Dec08. Building up on Supps again.

‹ Glucocorticoids Increase In Vitro and In Vivo Activities of Antibiotics [5] LPS protection -- Cheap! › [6]

If this is so, then I am a

Submitted by ruthless1 on Sun, 2008-04-20 18:10.

If this is so, then I am a bit confused.

The CPni [1] is hard to detect because of its 3 life phases & it may not be in the blood when you are aiming to see them.

that said, I thought they were harder to see & you had to have a BIG microscope???

Surprised

CFIDSi [7]/ME 25yrs, FMSi [8], IBSi [9], EBVi [10], Cpn, (insomnia - melatonini [11], GABA, tarazadone, triazolam, novocycloprine, allergy formula, 3 gm tryptophan), Natural HRT peri-M, NACi [12] 2.5 gm, 6-07 Doxy 200 mg day pm, Azith 375 mg M/W/Fday, Pulse8 750mg 4day,375X1 3-24-8

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CFIDSi [7]/ME 32 yrs, FMSi [8], IBSi [9], EBVi [10], CMV, Cpni [1], H1, chronic insomnia, Lymes, HME, Natural HRT peri-M, NAC 3 gm, Full CAP 6-2-08, all supplementsi [13] +Sea Kelp, Chitosan Pulse 16 1-4-09 1gm Flagyli [14]/day-3 days

»

 The picture on the home

Submitted by Jim K on Sun, 2008-04-20 19:35.

 The picture on the home page is taken with a Bradford microscope, a power usually not found in most office settings. Depnds on the power of the scope and the technique of the operator, as well as the number of EBi [15]'s in the blood.

CAPi [16] for Cpni [1] 11/04. Dx: 25yrs CFSi [17] & FMSi [8]. Protocol: 200mg Doxyi [3], 250mg MWF Azith, Tinii [18] 1000mg/day pulses; Vit D1000 units, Iodoral 50mg, T4 & T3

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CAPi [16] for Cpni [1] 11/04. Dx: 25yrs CFSi [17] & FMSi [8]. Currently: 150mg BID Roxithromycin, Doxycycline 100mg BID, Tinii [18] 1000mg/day pulses; Vit D2000 units, T4 & T3

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My doc looked at my blood

Submitted by sharon on Sun, 2008-04-20 21:22.

My doc looked at my blood but did not see CPNi [1]. He saw that my blood stuck together. Remember, cpn goes intracellulari [19].  The microscope does not look intracelluarly.  

Mphs, TN. CFSi [17], hypoT (Hashi), adrenal fatigue, 37 w/hormones of 80,. right arm neuropathy. + cpn, myco, EBVi [10], CMV. NACi [12] 4000mg, doxyi [3] 100-2xday, azith 250 m/w/f/sun, progesterone, estriol, synthroid, pulse flagyli [14], tinii [18]<

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Mphs, TN. CFSi [17], hypoT (Hashi), adrenal fatigue, 37 w/hormones of 80, right arm neuropathy. + cpni [1], myco, EBVi [10], CMV. Capi [16] began in 6/07. NACi [12] 2400mg, doxyi [3] 100-bid, biaxin 500mg bid since 7/08, progesterone, synthroid, flagyli [14] pulses

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Hi Theres an excellent book

Submitted by jean uk on Wed, 2008-04-23 15:37.

Hi Theres an excellent book by Lida Mattman who explains how to find the cell wall deficient bacteria and Dr AW learned how to find them through her I think

Jean 

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Jean, This book is really [20]

Submitted by Estrellia on Wed, 2008-04-23 15:48.

Jean,

This book is really expensive on Amazon , would it be available in a library?

I saw Dr AW last week and saw some of the "little critters" that were in my blood from the first slide straight away. He was going to check further slides after I had left for other signs of cause of infection because it can take a while to find them.  

Dawn

 

 

 

Dawn, Liverpool, UK, CAPi [16] for Chlamydia Pneumonia since Nov 2007. Amoxycillin 1000mg, Flagyl 400mg, Isoniazid 300mg. Also Azithromycn 125mg 3x per week.

2nd course , Ivermectin 12mgs x3, Metronidazolei [14] 400mg, Rifam

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Dawn, Liverpool, UK, CAPi [16] for Chlamydia Pneumonia since Nov 2007. Amoxycillin 1000mg, Flagyl 400mg, Isoniazid 300mg. Also Azithromycn 125mg 3x per week.

2nd course , Ivermectin 12mgs x3, Metronidazolei [14] 400mg, Rifam

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Dawn, Liverpool, UK, CAPi

Submitted by Estrellia on Wed, 2008-04-23 15:49.

Dawn, Liverpool, UK, CAPi [16] for Chlamydia Pneumonia since Nov 2007. Amoxycillin 1000mg, Flagyl 400mg, Isoniazid 300mg. Also Azithromycn 125mg 3x per week.

2nd course , Ivermectin 12mgs x3, Metronidazolei [14] 400mg, Rifam

___________________________________________________________

Dawn, Liverpool, UK, CAPi [16] for Chlamydia Pneumonia since Nov 2007. Amoxycillin 1000mg, Flagyl 400mg, Isoniazid 300mg. Also Azithromycn 125mg 3x per week.

2nd course , Ivermectin 12mgs x3, Metronidazolei [14] 400mg, Rifam

»

If your library hasn't got a

Submitted by speedbird on Wed, 2008-04-23 18:45.

If your library hasn't got a copy of this book they can get it for you through inter-library loans. There will probably be a small charge for this depending on the library reservation charging policy. Make sure that you have the full details including the ISBN and you should have no problem apart from the wait - not as long as the NHS though. Wink

New Forest, UK. Progressive MSi dx 12/06 LDNi [21] 3/07 CAPi [16] 6/07: Wheldon version.

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Progressive MSi [22] dx2006. LDNi [21] & CAPi [16]: Wheldon version. All supps. Doxyi [3] 200mg. Zithi [23] 250mg. Metroi [14] 400mg.Pulses #17...I can because I think I can.

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I had one done once but it

Submitted by clammed_up on Wed, 2008-05-21 12:53.

I had one done once but it was only alternative medicine  DR and done from a pin prick to the thumb. On the screen showed these strange balls floting around in my blood. They wernt red blood cells because the red blood cells appeared lighter and clear and these were more whitish but still round. The white blood cells were funy shaped so these wernt white blood cells either. The alternative med dr said he has no idea what these balls were and suggested maybe toxoplasmosis so i went to get checked for that... negative. Anyway, when i saw the pic of the cpni [1] on the opening page here I remembered thinking to myself.. holy cow those are the balls I saw on my dark field microscopy! But would cpn even be in blood from the fingertips? I guess if it lives in skin maybe???

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CPNi [1] pcri [2] and antibody positive , treating MSi [22], CFSi [17], TMJ, trigeminal neuralgia, IBSi [9] neutropenia, pus found in facial bone, Doxyi [3] 100x2,Doxy 200x2 zithro 250x1 alternate days. Metroi [14] pulses each month.

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My experience has been being

Submitted by Lisa B on Fri, 2008-05-23 09:09.

My experience has been being able to see the EBi [15]'s on my RBC's. The microscope my doc uses was custom made, he does the blood stick, prepares the slide and looks at the blood smear. He has a camera that can photograph the slide, this goes into the chart. I am very grateful to have this person as my doc. Managed care in the US has done a dis-service to folks with chronic illness. We also have to undergo diagnostic testing that is automated, no more looking under microscopes to do WBC counts due to the expense doc have in having to hire certified lab techs to do such things in doctor's offices. I could go on and on but better get off my soap box about all this.

Lisa B

ebvi [10],lymes, cpni [1] and or another gram+ bug; currently off capi [16] for now;Kiss

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  But as Ruthless said [24]

Submitted by Sarah on Fri, 2008-05-23 10:01.
 

But as Ruthless said above, CPni [1] has three life phases, so you won't always have EBs to see.  However powerful a dark field microscope, one has yet to be invented that can see the inside of a blood cell, so the other life phases will not be visible.

As an aside I bought Lida Mattman's book as a birthday present for DW a few years ago but it is rather disappointing being rather out of date now.  Oppenheimer's Diagnostic Neuropathology is much better, equally large and expensive and is kept up to date by Margaret Esiri, who succeeded Oppenheimer at Oxford.............Sarah

An Itinerary in Light and Shadow......................Completed Stratton/Wheldon regime for aggressive secondary progressive MSi [22] in June 2007, after nearly four years, three of which intermittent.   Still slowly improving and no exacerbation since starting. EDSSi [25] was 7, now 2, less on a good day.

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Completed Stratton/Wheldon regime for aggressive secondary progressive MSi [22] in June 2007, after nearly four years, three of which intermittent.   Still slowly improving and no exacerbation since starting. EDSSi [25] was 7, now 2, less on a good day.

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For some interesting pic's

Submitted by Jim K on Fri, 2008-05-23 18:51.

For some interesting pic's of Chlamydia inclusions by a highly technical but visible method see:

http://www.biomedcentral.com/1471-2334/6/165 [26]

 

These researchers are on the cutting edge of Chlamydia microbiology.

CAPi [16] for Cpni [1] 11/04. Dx: 25yrs CFSi [17] & FMSi [8]. Protocol: 200mg Doxyi [3], 300mg Roxithromycin, Tinii [18] 1000mg/day pulses; Vit D1000 units, Iodoral 50mg, T4 & T3

___________________________________________________________

 

CAPi [16] for Cpni [1] 11/04. Dx: 25yrs CFSi [17] & FMSi [8]. Currently: 150mg BID Roxithromycin, Doxycycline 100mg BID, Tinii [18] 1000mg/day pulses; Vit D2000 units, T4 & T3

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Now we can see the little

Submitted by speedbird on Fri, 2008-05-23 19:14.

Now we can see the little villains in their nests, this will help anyone who uses visualisation techniques to zoom in on their enemy. Images duly saved for future reference, thanks Jim. 

New Forest, UK. Progressive MSi dx 12/06 LDNi [21] 3/07 CAPi [16] 6/07: Wheldon version. Pulses so far #10

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Progressive MSi [22] dx2006. LDNi [21] & CAPi [16]: Wheldon version. All supps. Doxyi [3] 200mg. Zithi [23] 250mg. Metroi [14] 400mg.Pulses #17...I can because I think I can.

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Source URL (retrieved on 01/08/2009 - 7:31am): http://www.cpnhelp.org/finding_cpn_dark_field_mi

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[1] http://www.cpnhelp.org/glossary/term/167
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[3] http://www.cpnhelp.org/taxonomy/term/39
[4] http://www.cpnhelp.org/taxonomy/term/38
[5] http://www.cpnhelp.org/glucocorticoids_increase_
[6] http://www.cpnhelp.org/lps_protection_cheap
[7] http://www.cpnhelp.org/glossary/term/164
[8] http://www.cpnhelp.org/taxonomy/term/24
[9] http://www.cpnhelp.org/taxonomy/term/19
[10] http://www.cpnhelp.org/glossary/term/120
[11] http://www.cpnhelp.org/taxonomy/term/128
[12] http://www.cpnhelp.org/chlamydia_pneumoniae/supp
[13] http://www.cpnhelp.org/taxonomy/term/63
[14] http://www.cpnhelp.org/taxonomy/term/44
[15] http://www.cpnhelp.org/taxonomy/term/46
[16] http://www.cpnhelp.org/glossary/term/168
[17] http://www.cpnhelp.org/glossary/term/163
[18] http://www.cpnhelp.org/chlamydia_pneumoniae/an_0
[19] http://www.cpnhelp.org/glossary/term/114
[20] http://www.cpnhelp.org/print/4245#comment-31296
[21] http://www.cpnhelp.org/glossary/term/170
[22] http://www.cpnhelp.org/taxonomy/term/6
[23] http://www.cpnhelp.org/taxonomy/term/41
[24] http://www.cpnhelp.org/print/4245#comment-33449
[25] http://www.cpnhelp.org/glossary/term/171
[26] http://www.biomedcentral.com/1471-2334/6/165