Evidence of Systemic Dissemination of Chlamydia pneumoniae via Macrophages in the Mouse

An old one (1998) but still a good (IMOi) reference:

Evidence of Systemic Dissemination of Chlamydia pneumoniae via Macrophages
in the Mouse
<

Chlamydia pneumoniae has been postulated to cause systemic disease by infection of monocytes/
macrophages and spread via the blood or lymphatics. To investigate how C. pneumoniae disseminates,
the ability of the organism to infect murine macrophages in vivo and whether infection can
be transferred via macrophages were determined. C. pneumoniae was detected by direct plating,
isolation, and polymerase chain reaction in alveolar macrophages from intranasally inoculated
mice and peritoneal macrophages from intraperitoneally inoculated mice. C. pneumoniae were also
detected in peripheral blood mononuclear cells, but not plasma, of intranasally and intraperitoneally
inoculated mice. When alveolar or peritoneal macrophages were adoptively transferred by intraperitoneal
injection from infected to uninfected mice, C. pneumoniae DNA was detected by polymerase
chain reaction in lung, thymus, spleen, and/or abdominal lymph nodes. These results demonstrate
the ability of C. pneumoniae to infect macrophages in vivo and to disseminate systemically via
infected macrophages by hematogenous and lymphatic routes.

JeanneRoz